艾美捷 RmesFect CRISPR转染试剂，无需核吸收，蛋白表达速度快

高效：在所有细胞中实现RNA递送

即用型：无需额外的缓冲液

低核酸量：毒性小化

保护 RNA 免受降解

与任何培养基兼容

血清兼容：无需更换培养基

RmesFect™ CRISPR transfection Reagent based on the TEE-technology is specifically designed for mRNA/gRNA transfection with high efficiency ａnd low toxicity. RmesFect™ is efficient in a large variety of cells.

Benefits:

Highly efficient: achieve RNAs delivery in all cells

Ready-to-use: no need for additional buffer

Low nucleic acid amount : minimized toxicity

Protects RNAs against degradation

Compatible with any culture medium

Serum compatible : Medium changed not required

与质粒DNA递送相比，mRNA转染具有以下优势：

无需核吸收。mRNA翻译成蛋白质发生在细胞质中。

蛋白表达速度快于DNA转染

无基因组整合

以完全启动子非依赖的方式表达蛋白质

RmesFect CRISPR转染试剂结果示意图：

根据标准方案，使用RmesFect CRISPR用编码GFP和RFP的mRNA（各0.25µg）转染DC24细胞。转染后24小时在显微镜荧光下分析图像。

来源：https://www.amyjet.com/products/OZB-RMC70500.shtml