中英文说明丨艾美捷无RNase的DNase I试剂盒

Norgen＇s RNA purification kits isolate total RNA with minimal amounts of genomic DNA contamination. However, for some sensitive downstream applications, it may be desirable to remove all traces of residual DNA. Norgen＇s RNase-free DNAse I Kit, with Enzyme Incubation Buffer, can be used for optional on-column DNase digestion with any of Norgen＇s RNA purification kits. Alternatively, after isolating total RNA using one of Norgen＇s RNA purification kits, the RNA elution can be treated with this DNase I. The RNA can then be purified from the DNase using Norgen＇s RNA Clean-Up ａnd Concentration Kit (Cat# 23600), ａnd the RNA can then be used in downstream applications.

Each RNase-Free DNase I Kit is supplied complete with sufficient enzyme ａnd enzyme incubation buffer for 50 or 200 reactions.

For DNase digestion during RNA purification either on-column or in-solution

Convenient optimized on-column DNase treatment using Norgen＇s RNA Purification Kits

Also includes protocol for digestion in-solution followed by RNA Clean-Up

Guaranteed RNase-Free

Includes Enzyme Incubation Buffer

Cat. 25710 contains one vial of 1,600 units ａnd Cat. 25720 contains 4 vials (1,600 units/vial)

Storage Conditions

The DNase I provided is in lyophilized form. It is stable for at least 3 months if stored at room temperature. However, it is recommended to store the DNase I vial at 2 – 8ºC (or below) upon receipt to maintain stability beyond 3 months. Buffer DR ａnd Enzyme Incubation Buffer can be stored at room temperature. After reconstitution with Buffer DR (see product manual), the DNase I should be stored at -20ºC. All reagents should remain stable for at least 1 year in their unopened containers at the appropriate storage temperature.

艾美捷Norgen无RNase的DNase I试剂盒#NGB-25710：可分离出总RNA，而基因组DNA污染量少。然而，对于一些敏感的下游应用，可能需要去除所有残留DNA的痕迹。Norgen的无RNaseDNA酶I试剂盒带有酶孵育缓冲液，可用于使用Norgen的任何RNA纯化试剂盒进行可选的柱上DNase酶消化。或者，在使用Norgen的RNA纯化试剂盒分离总RNA后，可以用该DNase I处理RNA洗脱。然后可以使用Norgen的RNA净化和浓缩试剂盒（Cat# 23600）从DNA酶中纯化RNA，然后RNA可用于下游应用。

无RNase的DNase I试剂盒特点：

使用Norgen的RNA纯化试剂盒方便地优化了柱上DNase处理；还包括在溶液中消化的方案，然后是RNA清洁保证无RNase；包括酶培养缓冲液；#25710含有一小瓶1600单位和#25720包含4个小瓶（1600个单位/小瓶）。

储存条件：

提供的脱氧核糖核酸酶是冻干形式。如果在室温下储存，它可以稳定至少 3 个月。但是，建议在收到时将 DNase I 小瓶储存在 2 – 8ºC（或更低）的温度下，以保持稳定性超过 3 个月。缓冲液DR和酶孵育缓冲液可在室温下储存。用缓冲液DR复溶后DNase I应储存在-20ºC。所有试剂应在适当的储存温度下在其未开封的容器中保持稳定至少 1 年。

来源：https://www.amyjet.com/products/NGB-25710.shtml