艾美捷HRP标记亲和纯化山羊抗兔IgG（H+L）二抗中英文介绍

英文名字：Horseradish Peroxidase-conjugated AffiniPure Goat Anti-Rabbit IgG （H+L）

编号：111-035-003

规格：2ml

应用类型：WB（1:10000 - 1:200000）、ELISA（1:5000 - 1:100000） IHC（1:500 - 1:5000）

免疫原：兔IgG

来源宿主：山羊

反应性：该山羊抗兔二抗与兔IgG分子有特异性反应，与其它兔免疫球蛋白（包括IgM、IgE、IgD、IgA等）的轻链有交叉反应。该二抗与血清内非免疫球蛋白无交叉反应，但可能与其它种属的免疫球蛋白有交叉反应。

Normal serums are lipid extracted to improve clarity, dialyzed against phosphate buffered saline (PBS) containing sodium azide, ａnd freeze dried. Normal serum diluted to 5% (v/v) in PBS is strongly recommended as a blocking reagent to reduce background from non-specific, conserved sequence and/or Fc receptor binding. Best results are obtained with diluted normal serum from the same host as the labeled antibody, as a separate incubation step before addition of the primary antibody.

Physical State: Freeze-dried solid

Storage ａnd Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) ａnd centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.

Extended Storage after Rehydration: Aliquot ａnd freeze undiluted product at -20°C or below. Avoid repeated freezing ａnd thawing.

Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

Preservative: 0.05% Sodium Azide

Suggested Working Concentration or Dilution Range:

For blocking purposes, use 5% (v/v) solution (1:20 dilution from rehydrated volume). Rehydration according to instruction yields 100% serum.

对正常血清进行脂质提取以提高清晰度，用含有叠氮化钠的磷酸盐缓冲盐水（PBS）透析，并冷冻干燥。强烈推荐在PBS中稀释至5%（v/v）的正常血清作为阻断试剂，以减少非特异性、保守序列和/或Fc受体结合的背景。作为添加第一抗体之的单独培养步骤，用来自与标记抗体相同宿主的稀释的正常血清获得非常好的结果。

艾美捷HRP标记亲和纯化山羊抗兔IgG（H+L）二抗#111-035-003，基于免疫电泳和/或ELISA，抗体与全分子兔IgG反应。它还与其他兔免疫球蛋白的轻链发生反应。未检测到针对非免疫球蛋白血清蛋白的抗体。抗体可以与来自其他物种的免疫球蛋白发生交叉反应。

通过免疫亲和层析从抗血清中分离出完整的IgG抗体分子。它们具有通过二硫键连接在一起的Fc部分和两个抗原结合Fab部分，因此它们是二价的。据报道，平均分子量为约160kDa。整个IgG形式的抗体适用于大多数免疫检测程序，并且是具成本效益的。

来源：https://www.amyjet.com/products/111-035-003.shtml