中英文说明丨艾美捷含Ca++和Mg++的明胶弗洛拿缓冲液（GVB）

Aimijie Gelatin Flora Buffer with Ca++and Mg++:

Cat B102-250mL;  B100-1L

Name GVB++(with Ca++and Mg++) Gelatin Flora Buffer Containing Ca++and Mg++

Ingredients: 0.1% gelatin, 5mM Vernal, 145mM NaCl, 0.025% NaN3, pH7.3 Containing 0.15mM calcium chloride ａnd 0.5mM magnesium chloride

The application should use GVB++to prepare antibody sensitized sheep red blood cells (EA) ａnd clean them before use for testing; GVB++can be used to dilute all components of complement classical pathway ａnd lectin pathway experiments.

Concept:

During the dissolution process, two types of changes occur: one is the diffusion of solute molecules (or ions) into water under the action of water molecules, which absorbs heat; the other is the interaction of solute molecules (or ions) with water molecules to generate water ａnd molecules (or water ａnd ions), which release heat.

There are two changes during the dissolution process of substances:

Diffusion process: The diffusion of solute molecules (or ions) into water is a physical process that absorbs heat

Hydration process: the molecules (or ions) of solute interact with water molecules to generate hydrated molecules (or hydrated ions), which is a chemical process ａnd emits heat

Temperature changes during dissolution process:

a. The heat absorbed during the diffusion process is greater than the heat released during the hydration process, resulting in a decrease in solution temperature, such as NH4NO3 dissolved in water.

b. The heat absorbed during the diffusion process is less than the thermal vibration released during the hydration process, ａnd the solution temperature increases, such as NaOH or concentrated sulfuric acid dissolved in water.

c. The heat absorbed during the diffusion process ≈ the heat released during the hydration process, ａnd the solution temperature remains almost constant, such as NaOl dissolved in water.

Common frozen mixtures:

Ice is commonly used as a coolant in production ａnd daily life, but it can only provide a low temperature of around 0 ℃. Some medical ａnd research institutions often require strict ａnd low temperatures to provide convenient access to low temperatures

The method is to use a frozen mixture.

明胶弗洛拿缓冲液(Gelatin Veronal Buffer, GVB)和弗洛拿缓冲的生理盐水(Veronal Buffered Saline, VBS)是补体研究的必备缓冲液, 弗洛拿缓冲的生理盐水(VBS)和明胶弗洛拿缓冲液(GVB)相比缺少明胶组分。   

艾美捷含Ca++和Mg++的明胶弗洛拿缓冲液：

Cat B102-250mL; B100-1L

名称 GVB++ (with Ca++ ａnd Mg++)含Ca++和Mg++的明胶弗洛拿缓冲液

成分 0.1% gelatin, 5mM Veronal, 145mM NaCl, 0.025% NaN3 , pH7.3. Containing 0.15mM calcium chloride ａnd 0.5mM magnesium chloride

应用 应使用GVB++制备抗体致敏羊红细胞(EA)，并在用于测定对其进行清洗；GVB++可用于稀释补体经典途径和凝集素途径试验的所有成分。

概念：

在溶解过程中发生了两种变化，一种是溶质的分子（或离子）在水分子的作用下向水中扩散，这一过程吸收热量：另一种是扩散的溶质的分子（或离子）和水分子作用，生成水和分子（或水和离子），这一过程放出热量。

物质溶解过程中的两种变化：

扩散过程：溶质的分子（或离子）向水中扩散，是物理过程，吸收热量

水合过程：溶质的分子（或离子）和水分子作用，生成水合分子（或水合离子），是化学过程，放出热量

溶解过程中的温度变化：

a. 扩散过程中吸收的热量>水合过程中放出的热量，溶液温度降低，如：NH4NO3溶解于水。

b. 扩散过程中吸收的热量<水合过程中放出的热振，溶液温度升高，如：NaOH、浓硫酸溶解于水。

c. 扩散过程中吸收的热量≈水合过程中放出的热量，溶液温度几乎不变，如：NaOl溶解于水。

常见冷冻混合物：

在生产、生活中常用冰作冷却剂，但冰只能提供，0℃左右的低温。一些医疗和研究单位常需要严低的温度，提供低温较方便的

方法是用冷冻混合物。

下面是几种常见冷冻混合物的组成及所能达到的低温度。

冷冰混合物的组成 低温度

1gH4NO3和100g冰

19MH4C1和100g冰 -16

23gNaCl和100g冰 -21

22gMgC12和100g冰 -34

来源：https://www.amyjet.com/products/B102.shtml