解决方案丨艾美捷萤火虫荧光素酶测定试剂盒

艾美捷萤火虫荧光素酶测定试剂盒2.0,150次测定#BTM-30085-1组件：

5X 萤火虫萤光素酶裂解缓冲液

萤火虫萤光素酶检测缓冲液 2.0

D-荧光素

萤火虫荧光素酶测定试剂盒相关特征：

高灵敏度和线性闪光型测定

每次检测都添加新鲜的底物，以确保信号随时间推移保持一致

高性价比

该萤火虫荧光素酶测定试剂盒属性：

化验类型/选项：终点测定，发光（闪光型）

检测方法/读数：光度计（带试剂注射器的单管或微量板读取器）

储存条件：在-60°C或更低温度下储存

Firefly Luciferase is widely used as a reporter gene to study gene regulation ａnd function ａnd drug screening. Since there is no endogenous Luciferase activity in mammalian cells or tissues, it is a very sensitive genetic reporter gene. Firefly Luciferase is a 62000 Dalton protein, which is active as a monomer, ａnd does not require subsequent processing of its activity. This enzyme catalyzes the oxidation of ATP dependent D-luciferin to oxidized luciferin, producing light emission. The firefly Luciferase follows the Michaelis Menten kinetics, so the maximum light output can be achieved only when the substrate ａnd cofactor are in large excess. When analyzing under these conditions, the light emitted by the reaction is proportional to the number of Luciferase molecules.

Note: This is a flash type luminescence detection that requires the signal to be measured immediately after adding the working solution to the sample. It requires a single tube luminescent meter or luminescent enzyme reader with a reagent syringe. Biotium also offers the Steady Luc HTS Firefly detection kit (product number 30028L), which is a homogeneous luminescent detection with a signal half-life of 3-5 hours.

Easy to use for consistent results

The firefly Luciferase detection kit is designed to simply ａnd effectively quantify the activity of firefly Luciferase reporter enzyme in cultured cells, with high sensitivity ａnd linearity. This kit is a new improved substitute for the Firefly Luciferase Test Kit (Catalog No. 30003). It adopts Firefly Assay Buffer 2.0, which has better working solution stability compared to the original detection buffer.

Other commercial Luciferase tests require you to dilute all firefly substrates in the test buffer, resulting in a limited shelf life after first use. In Biotium＇s reagent kit, the substrate is provided as a separate stock solution, which is freshly diluted for each test to achieve excellent shelf life ａnd provide consistent signals over time.

The measurement size is based on the 96 hole format. This kit includes sufficient cell lysis buffer to perform the specified number of measurements within the size range of 96 well to 24 well plates.

来源：https://www.amyjet.com/products/BTM-30085-1.shtml