解决方案丨艾美捷海肾荧光素酶测定试剂盒

艾美捷海肾荧光素酶测定试剂盒2.0,150次测定#BTM-30082-1组件：

5X 被动裂解缓冲液

海肾萤光素酶检测缓冲液 2.0

Aquaphile™ 腔肠素

海肾荧光素酶测定试剂盒相关特征：

高灵敏度和线性快速型 RLuc 测定

易于处理的水溶性基材

高性价比

海肾荧光素酶测定试剂盒属性：

化验类型/选项：终点测定，发光（闪光型）

检测方法/读数：光度计（带试剂注射器的单管或微量板读取器）

储存条件：在-60°C或更低温度下储存

Sea kidney Luciferase has been used as a reporter gene to study gene regulation ａnd function in vitro ａnd in vivo. It is usually used for multiple transcriptional reporter gene detection or as a standardized transfection control for firefly Luciferase detection. Renila Luciferase is a 36000 Dalton monomer protein that catalyzes the oxidation of coelenterazine by oxygen to produce light. The activity of this enzyme does not require post translational modification ａnd can serve as a genetic reporter gene immediately after translation. Coelenterazine also emits light through enzyme independent oxidation, a process called self luminescence. Superoxide anions ａnd peroxynitrite in cells ａnd tissues enhance self luminescence. This test kit uses a special buffer formula, which aims to conduct reliable linear measurement of Luciferase activity in the sea ａnd kidney with the minimum self luminous background ａnd excellent sensitivity.

Note: This reagent kit is a flash type luminescence detection that requires immediate measurement of luminescence after adding reagents to your sample. It requires a single tube luminescent meter or a luminescent enzyme-linked immunosorbent assay instrument with a reagent syringe.

Easy to use for consistent results

This kit is a new improved substitute for Haishen Luciferase Test Kit (Catalog No. 30004). In addition to providing a simplified experimental scheme, Renilla Luciferase Test Kit 2.0 also has Biotium Aquaphile ™  Coelenterazine, a water-soluble substrate, is different from coelenterazine＇s methanol solution. It can be stored at - 20 ° C with the minimum evaporation.

The measurement quantity is based on a 96 well measurement format. This kit contains sufficient passive lysis buffer to perform the specified number of measurements within the plate size range of 96 to 24 wells.

来源：https://www.amyjet.com/products/BTM-30082-1.shtml