均相、高灵敏度艾美捷Firefly HTS检测试剂盒

Biotium的Steady-Luc™HTS检测系统是一种有的混合物，可通过防止在酶表面形成腺苷酰氧化荧光素来改变酶促反应，从而产生持久的信号（稳定发光）。 它是一种同质的高灵敏度萤火虫荧光素酶报告基因测定试剂盒，具有约3小时的半衰期，用于定量哺乳动物细胞中的萤火虫荧光素酶表达，允许在微孔板中进行批量处理。与闪光型检测相比，发光型荧光素酶检测（如Steady-Luc™）的发光信号较低。 检测的灵敏度和限将取决于实验系统中的荧光素酶表达水平以及光度计灵敏度。

Biotium还提供Steady-Luc™Firefly HTS检测试剂盒（冻干），冻干检测缓冲液便于室温运输和-20°C储存。

艾美捷Firefly HTS检测试剂盒#BTM-30028-1是一种均相、高灵敏度的萤火虫荧光素酶报告基因检测试剂盒。它是一种发光型检测，信号半衰期约为 3 小时，可在微孔板中进行批处理。

组件：

D-荧光素

Steady-Luc™ 萤火虫检测缓冲液

特征：

简单的单步均相测定

发光半衰期约3小时

物超所值：与其他品牌相比节省高达45%

Firefly HTS检测试剂盒属性：

化验类型/选项：高通量分析、均质分析、发光（发光型）

检测方法/读数：微孔板读取器（发光）

储存条件：在-60°C或更低温度下储存

Firefly Luciferase is widely used as a reporter gene to study gene regulation ａnd function ａnd drug screening. Due to the lack of endogenous activity in mammalian cells or tissues, it is a highly sensitive genetic reporter gene. However, the light generated by the reaction leads to the formation of suicidal adenosyloxyfluorescein on the surface of the enzyme. It results in a very short half-life of light emission with flashing dynamics. Because of this, flash type luminescence must be detected immediately after the reaction begins, which is incompatible with batch analysis of samples.

Steady Luc from Biotium ™  The Firefly HTS detection kit contains a proprietary mixture of substances that can prevent adenosine oxyfluorescein reactions ａnd generate persistent luminescent signals. It generates a luminescent signal with a half-life of approximately 3 hours for high-throughput microplate detection.

Compared to flash type determination, Steady Luc ™  Isoluminescent Luciferase assay has low luminescence signal. The sensitivity ａnd detection limit of this determination will depend on the expression level of Luciferase in your experimental system ａnd the sensitivity of the photometer.

来源：https://www.amyjet.com/products/BTM-30028-1.shtml