补体研究bi备缓冲液丨艾美捷多种明胶弗洛拿缓冲液(GVB)

Gelatin Verona Buffer (GVB) ａnd Verona Buffered Saline (VBS) are essential buffers for complement research. Compared to Gelatin Verona Buffer (GVB), Verona Buffered Saline (VBS) lacks gelatin components.

Gelatin Verona Buffer (GVB):

B102-250mL; B100-1L

Name GVB++(with Ca++and Mg++) Gelatin Flora Buffer Containing Ca++and Mg++

Ingredients: 0.1% gelatin, 5mM Vernal, 145mM NaCl, 0.025% NaN3, pH7.3 Containing 0.15mM calcium chloride ａnd 0.5mM magnesium chloride

The application should use GVB++to prepare antibody sensitized sheep red blood cells (EA) ａnd clean them before use for testing; GVB++can be used to dilute all components of complement classical pathway ａnd lectin pathway experiments.

B103-250mL; B101-1L

Name GVBo (GVB without Ca++and Mg++) Gelatin Flora Buffer without Ca++and Mg++

Ingredients: 0.1% gelatin, 5mM Vernal, 145mM NaCl, 0.025% NaN3, pH7.3

Apply GVBo to prepare rabbit red blood cells (Er) ａnd clean them before use for assay; GVBo can be used to dilute all components of the complement bypass pathway test.

B104-250mL; B105-1L

Name GVBE (GVBo with EDTA) Gelatin Flora Buffer with EDTA

Ingredients: 0.1% gelatin, 5mM Vernal, 145mM NaCl, 0.025% NaN3, 10mM EDTA, pH7.3

The application of GVBE is usually used as a buffer added at the end of the assay to prevent complement activation ａnd dilute the reaction to a level that can be read by spectrophotometry. It is also used to establish a control experiment where complement activation should not occur.

B110-250mL; B111-1L

Name SGVB++(with Ca++and Mg++) Gelatin Flora Buffer Containing Sucrose, Ca++, ａnd Mg++

Ingredients: 170mM Sucrose, 0.1% gelatin, 5mM Vernal, 57mM NaCl, 0.025% NaN3, 0.15mM calcium chloride, ａnd 0.5mM magnesium chloride, pH 7.3

SGVB++can be used to detect C1 in purified C1 complex (product number A098) ａnd normal human serum samples. In these experiments, all components were diluted in SGVB++and EA cells were washed into this buffer. In binding experiments, such as detecting the binding of C5, factor B, or factor H to EsC3b cells (sheep red blood cells with surface bound C3b) or ZymosanC3b cells, it also benefits from being performed in SGVB++. The combination of SGVB++can be enhanced as much as 10 times compared with the use of buffer solutions with physiological ionic strength (such as GVB or PBS).

明胶弗洛拿缓冲液(Gelatin Veronal Buffer, GVB)和弗洛拿缓冲的生理盐水(Veronal Buffered Saline, VBS)是补体研究的必备缓冲液, 弗洛拿缓冲的生理盐水(VBS)和明胶弗洛拿缓冲液(GVB)相比缺少明胶组分。   

艾美捷多种明胶弗洛拿缓冲液(Gelatin Veronal Buffer, GVB)：

编号 B102-250mL; B100-1L

名称 GVB++ (with Ca++ ａnd Mg++)含Ca++和Mg++的明胶弗洛拿缓冲液

成分 0.1% gelatin, 5mM Veronal, 145mM NaCl, 0.025% NaN3 , pH7.3. Containing 0.15mM calcium chloride ａnd 0.5mM magnesium chloride

应用 应使用GVB++制备抗体致敏羊红细胞(EA)，并在用于测定对其进行清洗；GVB++可用于稀释补体经典途径和凝集素途径试验的所有成分。

编号 B103-250mL; B101-1L

名称 GVBo (GVB without Ca++ ａnd Mg++)不含Ca++和Mg++的明胶弗洛拿缓冲液

成分 0.1% gelatin, 5mM Veronal, 145mM NaCl, 0.025% NaN3 , pH7.3.

应用 GVBo应用于制备兔红细胞(Er)，并在用于测定对其进行清洗; GVBo可用于稀释补体旁路途径试验的所有组分。

编号 B104-250mL; B105-1L

名称 GVBE (GVBo with EDTA)含EDTA的明胶弗洛拿缓冲液

成分 0.1% gelatin, 5mM Veronal, 145mM NaCl, 0.025% NaN3, 10mM EDTA, pH7.3.

应用 GVBE通常用作在测定结束时添加的缓冲液，以阻止补体活化并将反应稀释到可分光光度法读取的水平。它也用于建立不应发生补体活化的对照试验。

编号 B110-250mL; B111-1L

名称 SGVB++ (with Ca++ ａnd Mg++)含蔗糖，Ca++和Mg++的明胶弗洛拿缓冲液

成分 170mM Sucrose, 0.1% gelatin, 5mM Veronal, 57mM NaCl, 0.025% NaN3 , 0.15mM calcium chloride, ａnd 0.5mM magnesium chloride, pH7.3.

应用 SGVB++可用于检测纯化的C1复合物(货号A098)和正常人血清样品中的C1。在这些实验中，所有成分在SGVB++中稀释，并将EA细胞冲洗到该缓冲液中。在结合实验中，如检测C5、因子B或因子H与EsC3b细胞(带有表面结合的C3b的绵羊红细胞)或ZymosanC3b细胞的结合，也受益于在SGVB++中进行。SGVB++与使用具有生理离子强度的缓冲液(如GVB或PBS)相比，结合的增强可多达10倍。

来源：https://www.amyjet.com/brand/gvb.shtml