标题人丙二醛（MDA）试剂盒英文说明书

人丙二醛（MDA）试剂盒
英文名称：Human Malondialdehyde（MDA） KIT
FOR RESEARCH USE ONLY
Assay range：0.3 mmol/L -8 mmol/L                96 determinations
Purpose
This  kit allows  for  the determination  of MDA concentrations  in Human serum,  cell culture supernates ａnd other biological fluids
Principle of the assay
The kit assay Human MDA level  in the sample，use Purified Human MDA antibody  to coat microtiter plate wells, make solid-phase antibody, then add MDA to wells, Combined MDA antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after
washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction  is  terminated by  the addition of a sulphuric acid solution and  the color  change  is  measured  spectrophotometrically  at  a  wavelength  of  450  nm.  The concentration of Human MDA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Important notes
1. The kit  takes out  from  the  refrigeration environment should be balanced 15-30 minutes  in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
2. washing  buffer will Crystallization  separation,  it  can  be  heated  the  water  helps  dissolve when dilute . Washing does not affect the result.
3. add  Sample  with  sampler  Each  step,  And  proofread  its  accuracy  frequently,  avoids  the experimental error. add sample within 5 min, if the number of sample is much , recommend to use Volley .
4. if the testing material content is excessively higher （The sample OD is bigger than the first standard well ）,please dilute Sample （n-fold）, Please diluente ａnd multiplied by the dilution factor.（× n× 5）.
5. Closure plate membrane only limits the disposable use, to avoid cross-contamination.
6. The substrate evade the light preservation.
7. Please  according  to  use  instruction  strictly,  The  test  result  determination must  take  the microtiter plate reader as a standard.
8. All samples, washing buffer ａnd each kind of  reject should according  to  infective material process.
9. Do not mix reagents with those from other lots.
Storage ａnd validity
1．Storage：    2-8℃.
2．validity：  six months