标题干货|多肽溶解性指南

       确定溶解合成肽的*佳溶剂通常是一个挑战。虽然许多肽在水溶液中具有良好的溶解性，但也有其他多肽具有低溶解度和不溶性。当多肽具有长的疏水性氨基酸序列时，其溶解将会成为一个特别的挑战。以下指南我们将提供一些有助于预测肽溶解的建议。

1.It is best to aliquot ａnd test a small quantity of peptide first to determine optimum conditions.

先分装并进行少量多肽的溶解度测试，以确定*佳溶解条件。

2. Ensure you understand the appropriate concentration at which the peptide should be dissolved. Please see this link to a great molarity calculator（https://www.graphpad.com/quickcalcs/molarityform.cfm） to help you determine the proper concentration. 

     确保您了解多肽溶解的适当浓度，请查看 molarity calculator摩尔计算器（https://www.graphpad.com/quickcalcs/molarityform.cfm），以帮助您计算确定适当的浓度。

3. Depending on peptide composition, there are different solvents that are needed to dissolve your lyophilized peptide. Please confirm solubility with 2-3 different sources (publications, suppliers). 

      根据肽的组成，溶解冻干肽需要不同的溶剂。请确认2-3种不同来源（出版物，供应商）的溶解度。

4. Some general guidelines include:

1）Peptides shorter than five residues are usually soluble in water or aqueous buffer, except when the entire sequence consists of hydrophobic amino acids (e.g., W, L, I, F, M, V, Y).

2）Hydrophilic peptides containing > 25% charged residues (e.g., D, K, R, H ａnd E) ａnd 25% hydrophobic amino acids are usually soluble in water or aqueous buffers.

3）Peptides containing 50% ａnd more hydrophobic residues might be insoluble or only partly soluble in aqueous solutions.

多肽溶解一般准则包括：

1）短于5个残基的肽通常可溶于水或水性缓冲液，除非整个序列由疏水性氨基酸（例如W，L，I，F，M，V，Y）组成。

2）含有> 25％带电残基（例如D，K，R，H和E）和25％疏水性氨基酸的亲水性肽通常可溶于水或水性缓冲液中。

3）含有50％和更多疏水残基的肽可能不溶或仅部分溶于水溶液。

Please consult literature pertaining to your specific protein as the above are only general guidelines.

具体请参阅有关您的特定蛋白质/多肽的相关文献。

5. Warming your solution (< 40°C) may aid in dissolving your peptide. 

加热溶剂（<40°C）可能有助于溶解你的多肽。

6. The table below may help in determining the characteristics of your particular peptide. 

下表将有助于确定您目的肽的特征。