推荐：GranuCult® prime Hektoen Enteric Agar

别名:HE Agar, HEA, Hektoen Enteric Agar

属性
Agency
FDA-BAM
according to ISO 21567
according to ISO 6579

质量水平
200

sterilization compatibility
autoclavable compatible

形式
granular (dehydrated culture medium)

包装
pkg of 500 g

manufacturer/tradename
GranuCult® prime

储存条件
protect from light (Keep dry ａnd tightly closed)

technique(s)
microbiological culture: suitable

颜色
dark green

pH值（酸碱度）
7.5-7.9 (25 °C, 75 g/L in H2O)

溶解性
75 g/L

application(s)
clinical
food ａnd beverages
industrial qc
pharmaceutical
microbiology

储存温度
15-25°C

适用性
selective ａnd differential for Salmonella spp.
selective ａnd differential for Shigella spp.

说明
一般描述
GranuCult® prime Hektoen Enteric Agar provides good colonial differentiation ａnd inhibits some coliforms ａnd other non-lactose-fermenting bacteria, thereby facilitating the isolation ａnd differentiation of Salmonella spp. ａnd Shigella spp. from samples such as food chain ａnd other materials.Lactose-positive colonies have a different color from lactose-negative ones due to the presence of the two indicators for carbohydrate dissimilation: bromothymol blue ａnd acidic fuchsin. The two additional carbohydrates, sucrose (saccharose) ａnd salicin, also cause the color difference in colonies. The black color in the H2S-positive colonies is due to thiosulfate as a reactive compound with an iron salt as an indicator.This culture medium is moderately to highly selective. The mixture of bile salts suppresses the growth of most of the accompanying microorganisms. The inhibitory effect of bile salts on the growth of Shigella spp. is reduced by relatively large amounts of peptone ａnd carbohydrates, which allow good growth. Agar is the solidifying agent.
应用
GranuCult® prime Hektoen Enteric Agar is suitable for the isolation ａnd differentiation ofSalmonella ａnd Shigella from the food chain ａnd other materials.
特点和优势
GranuCult® offers superior granulated culture media.
Safe ａnd sustainable due to reduced risks associated with fine dust ａnd toxic substance inhalation, resulting in a safer work environment.
Excellent wettability, solubility, ａnd free-flowing properties
Convenient, with minimal component separation ａnd clumping, even under warm or humid conditions.
High batch-to-batch reproducibility
Prolonged shelf life of up to five years
High number of test strains exceeding all regulatory demands
Granulation technology allows many supplements to be included, with no need to add these separately
储存及稳定性
Self-prepared plates can be stored in the dark ａnd protected against evaporation:
acc. to ISO 21567 at (5 ± 3 °C) for up to 2 weeks
acc. to FDA-BAM at (4 ± 2 °C) for up to 30 days
acc to Corry et al., (2012) at (5 ± 3 °C) for up to 3 weeks
分析说明
Salmonella spp. produce transparent green to blue-green colonies, with or without black centers, or may appear completely black due to H2S production.
Shigella spp. produce green, transparent moist colonies.
Lactose, Sucrose or Salicin fermenting gram-negative bacteria may be inhibited or produce red to salmon colored colonies with or without precipitation zone.
其他说明
Do not use clumped or discolored medium.
The prepared medium is clear to slightly opalescent ａnd blue-green to blue-grey.
Before inoculation, allow the prepared medium to equilibrate at room temperature if it is stored at a lower temperature.
There should be no visible moisture on the plates before use. When moisture is present, the plates should be dried for the minimum time required to remove visible moisture, following the procedure as described by EN ISO 11133.
附注
We offer two media types: the superior granulated GranuCult® ａnd the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

IF<5，赠送价值 500元礼品卡
5 ≤IF≤10，赠送价值 1000元礼品卡
IF≥10，赠送价值 1000元礼品卡+任意 1 款 ValukineTM 试剂盒


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发表刊物        影响因子           奖励金额
SCI期刊杂志      1.0≤1F＜3.0          200元
SCI期刊杂志      3.0≤1F＜6.0          400元
SCI期刊杂志      6.0≤1F＜10.0         600元
SCI期刊杂志      1F≥10                1000元