VRB (Violet Red Bile Lactose)-MUG agar

别名:Coliform selective agar, MUG Violet Red Bile Agar, VRB-MUG agar, Violet Red Bile Agar with MUG, Violet Red Bile Lactose-MUG agar

属性
Agency
FDA-BAM

质量水平
200

形式
medium granules (dehydrated (DCM))

包装
pkg of 500 g

manufacturer/tradename
FluoroCult®

储存条件
(Keep tightly closed ａnd dry.)

technique(s)
microbiological culture: suitable

颜色
pink

pH值（酸碱度）
7.3-7.5 (25 °C, 39.6 g/L in H2O)

溶解性
39.6 g/L (after autoclaving)

堆积密度
620 kg/m3

application(s)
food ａnd beverages
microbiology

储存温度
15-25°C

适用性
Escherichia coli
coliforms

说明
一般描述
Fluorocult® VRB-MUG Agar is a selective medium for the detection ａnd enumeration of coliform bacteria, in particular E. coli. Meat peptone provides amino acids ａnd other complex nitrogenous substances, ａnd yeast extract supplies Vitamin B complexes. Lactose is a fermentable carbohydrate. Crystal violet ａnd bile salts inhibit the growth of accompanying Gram-positive bacterial flora. Neutral red acts as a pH indicator. Lactose-positive colonies show a color change to red due to acid production. Lactose-negative Enterobacteriaceae are colorless. Lactose-positive colonies are red ａnd often surrounded by a turbid zone due to the precipitation of bile acids. E. coli colonies appear fluorescent under UV light. β-D-glucuronidase, which is produced by E. coli, cleaves 4-methylumbelliferyl-β-D-glucuronide (MUG) to 4-methylumbelliferone ａnd glucuronide. The fluorogenic 4-methylumbelliferone can be detected under a long-wavelength UV lamp.
应用
Fluorocult® VRB Agar is suitable for the detection ａnd enumeration of coliform bacteria, particularly E. coli, in food ａnd dairy products.
特点和优势
Fluorocult® fluorogenic culture media offer Rapid ａnd Reliable culture testing
Powerful alternatives to conventional microbiological methods for the rapid ａnd sensitive detection of bacteria
Offers superior granulated culture media
Safe ａnd sustainable due to reduced risks associated with fine dust ａnd toxic substance inhalation, resulting in a safer work environment
Excellent wettability, solubility, ａnd free-flowing properties
Convenient, with minimal component separation ａnd clumping, even under warm or humid conditions
High batch-to-batch reproducibility
Prolonged shelf life of up to five years
High number of test strains exceeding all regulatory demands
分析说明
Appearance (clearness): clear
Appearance (color): dark red
pH-value (25 °C): 7.2 - 7.6
Solidification behaviour (2 hrs., 45 °C): liquid
Growth promotion test in accordance with the current version of DIN EN ISO 11133.

Growth(Escherichia coli ATCC 8739 (WDCM 00012)): good to very good
Growth Escherichia coliATCC 25922 (WDCM 00013): good to very good
Growth (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): no limit
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): total inhibition
Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087)): total inhibition
Colonies (Escherichia coli ATCC 8739 (WDCM 00012)): red with precipitation halo
Colonies (Escherichia coli ATCC 25922 (WDCM 00013)): red with precipitation halo
Colonies (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): colourless to beige
Fluorescence (Escherichia coli ATCC 8739 (WDCM 00012)): + (poor)
Fluorescence (Escherichia coli ATCC 25922 (WDCM 00013)): +
Fluorescence (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): + (greenish)
Incubation: 18 - 24 hours at 35 °C aerobic.

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发表刊物        影响因子           奖励金额
SCI期刊杂志      1.0≤1F＜3.0          200元
SCI期刊杂志      3.0≤1F＜6.0          400元
SCI期刊杂志      6.0≤1F＜10.0         600元
SCI期刊杂志      1F≥10                1000元