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别名:Perfringens selective agar, Perfringens selective agar acc. to ANGELOTTI, ANGELOTTI Perfringens selective agar, Sulfite Polymyxin Sulfadiazine Agar
属性
形式
medium granules (dehydrated (DCM))
质量水平
200
包装
pkg of 500 g
manufacturer/tradename
GranuCult®
储存条件
dry media (Keep tightly closed)
technique(s)
microbiological culture: suitable
颜色
light brown
pH值(酸碱度)
6.9-7.1 (35 °C, 40 g/L in H2O, after autoclaving)
溶解性
40 g/L
堆积密度
580 kg/m3
application(s)
food and beverages
microbiology
储存温度
15-25°C
适用性
Clostridium perfringens
Clostridium spp.
说明
一般描述
Sulfite Polymyxin Sulfadiazine (SPS) Agar contains a broad spectrum of nutrients. Most clostridia (including C. perfringens) reduce sulfite to sulfide, which reacts with iron citrate and causes the colonies to turn black. Other sulfite-reducing microorganisms are suppressed by polymyxin and sulfadiazine (sulfapyrimidine). The low sulfite content allows the growth of even sulfite-sensitive clostridia, which exhibit an adequate blackening of the colonies.
应用
SPS agar is suitable for the isolation and enumeration of Clostridium perfringens and Clostridium botulinum in all types of foodstuffs.
特点和优势
GranuCult® offers superior granulated culture media
Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
Excellent wettability, solubility, and free-flowing properties
Convenient, with minimal component separation and clumping, even under warm or humid conditions
High batch-to-batch reproducibility
Prolonged shelf life of up to five years
High number of test strains exceeding all regulatory demands
Granulation technology allows many supplements to be included, with no need to add these separately
分析说明
Appearance (clearness): clear
Appearance (colour): yellowish-brown
pH-value (25 °C): 6.8 - 7.2
Growth (Clostridium perfringens ATCC 10543): good to very good
Growth (Clostridium perfringens ATCC 13124 (WDCM 00007)): good to very good
Growth (Clostridium sporogenes ATCC 11437): good to very good
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): to fair
Growth (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): to poor
Blacking (Clostridium perfringens ATCC 10543): +
Blacking (Clostridium perfringens ATCC 13124 (WDCM 00007)): +
Blacking (Clostridium sporogenes ATCC 11437): +
Blacking (Escherichia coli ATCC 25922 (WDCM 00013)): -
Blacking (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): -
Incubation: 48 hrs.; 35 °C; aerobic.
附注
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
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