标题Caspase 1 antibody

提供者：上海笃玛生物科技有限公司发布时间：2024/8/13 阅读次数：23次 >>进入该公司展台

Product Name	Caspase 1 antibody
Antibody Type	Primary Antibodies
Product description	Rabbit Polyclonal antibody to Caspase 1 alpha (caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase))This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large ａnd small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave ａnd activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, ａnd wound healing. This gene has been shown to induce cell apoptosis ａnd may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing of this gene results in five transcript variants encoding distinct isoforms. [provided by RefSeq] Hide
Immunogen	Recombinant protein encompassing a sequence within the center region of human Caspase 1. The exact sequence is proprietary.

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Applications	ICC/IFIHC-PWB
	*Optimal dilutions/concentrations should be determined by the researcher. :
Species Reactivity	Human
Concentration	1 mg/ml
Purification	Affinity purified

Gene Synonyms	ICE antibody IL1BC antibody P45 antibody CASP1 antibody CASP1 nirs variant 1 antibody IL-1BC antibody caspase-1 antibody interleukin 1-B converting enzyme antibody IL1B-convertase antibody IL-1 beta-converting enzyme antibody "caspase 1 apoptosi Hide
Molecular Weight(MW)	45 kDa (note) (4)
Cellular Localization	Cylasm

Application

Immunofluorescence analysis of methanol-fixed HeLa, using Caspase 1 alpha antibody at 1:500 dilution.
Application

Treated (+) THP-1 conditioned medium (30 µg) was separated by 15% SDS-PAGE, ａnd the membrane was blotted with Caspase 1 antibody [N1N3] diluted at 1:1000.
Application

Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE diluted at 1:5000
Application

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Caspase 1 alpha antibody at 1:100 dilution.

Positive Control	A549 , HeLa , HepG2 , HCT116 , THP-1 conditioned medium (500 ng/ml LPS treatment for 4 hr ａnd 1 mM ATP treatment for 1.5hr)
Application Notes	*Optimal dilutions/concentrations should be determined by the researcher. :

Form	Liquid
Storage Instructions	Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer	1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

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