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| Product Name | NRF2 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NRF2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPICC/IFIHC-PIPWB |
| WB:1:200~1:1000 More IP:1:50~1:200 ICC/IF:1:50~1:250 IHC-p:1:25~1:100 | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Rhesus Monkey, BovineRhesus Monkey (99%), Bovine (84%) |
| Concentration | 0.5 mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Hide |
|---|---|
| Molecular Weight(MW) | 68 kDa (note) (8) |
| Cellular Localization | Cylasm , cytosol , Nucleus |
NRF2 antibody [N2C2], Internal detects NRF2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NRF2 protein stained by NRF2 antibody [N2C2], Internal diluted at 1:200. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.
Immunoprecipitation of NRF2 protein from HepG2 whole cell extracts using 5 μg of NRF2 antibody [N2C2], Internal . Western blot analysis was performed using NRF2 antibody [N2C2], Internal . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
ChIP was performed with HepG2 chromatin extract ａnd 5 μg of either normal rabbit IgG or anti-NRF2 antibody. The precipitated DNA was detected by PCR with primer set targeting to GCLC gene locus.
Immunohistochemical analysis of paraffin-em
NRF2 antibody [N2C2], Internal detects NRF2 protein at cylasm ａnd nucleus by immunofluorescent analysis. Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NRF2 protein stained by NRF2 antibody [N2C2], Internal diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
NRF2 antibody detects NRF2 protein by western blot analysis. Non-transfected (-) ａnd NRF2-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, ａnd the membrane was blotted with NRF2 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Whole cell extract (30 µg) was separated by 5% SDS-PAGE, ａnd the membrane was blotted with NRF2 antibody [N2C2], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Untreated (–) ａnd treated (+) MDA-MB-231 nuclear extracts (30 µg) were separated by 7.5% SDS-PAGE, ａnd the membrane was blotted with NRF2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
| Positive Control | MDA-MB-231 NE , MDA-MB-231 NE treated tBHQ , 3xFlag-human NFE2L2-transfected 293T , C8D30 |
|---|---|
| Application Notes | WB:1:200~1:1000 Hide IP:1:50~1:200 ICC/IF:1:50~1:250 IHC-p:1:25~1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
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