标题CD31 antibody Catalog Number：OM244946

提供者：上海笃玛生物科技有限公司发布时间：2024/8/20 阅读次数：14次 >>进入该公司展台

Product Name	CD31 antibody
Antibody Type	Primary Antibodies
Product description	Mouse monoclonal antibody [HEC7] to CD31The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, ａnd some types of T-cells, ａnd makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily ａnd is likely involved in leukocyte migration, angiogenesis, ａnd integrin activation. [provided by RefSeq, May 2010] More
Immunogen	Human PECAM-1

Clonality	Monoclonal
Isotype	IgG2a
Clone Number	HEC7
Host Species	Mouse
Tested Applications	ELISAFACSGSAICC/IFIHCIPWB
	WB:1:500~1:2000 IP:1:100~1:500 ICC/IF:1:50~1:100 IHC:1:50~1:200 Hide
Species Reactivity	BovineHumanRat
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration)
Purification	Protein G

Gene Synonyms	platelet/endothelial cell adhesion molecule 1 CD31 endoCAM PECA1 GPIIA＇ CD31/EndoCAM PECAM-1 PECAM1 Hide
Target	CD31

Application

FACS analysis of PBMC cells using CD31 antibody [HEC7] (green) or an isotype control (blue).
Application

FACS analysis of PBMC cells using CD31 antibody [HEC7].
Application

Flow cytometry analysis of CD31 in BAEC cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde ａnd washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature ａnd incubated with CD31/PECAM-1 antibody [HEC7] at a dilution of 0.5 ug/test for 60 min at room temperature. Cells were then incubated in a proper secondary antibody ａnd re-suspended in PBS for FACS analysis.
Application

Immunofluorescent analysis of CD31 (green) in THP-1 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes ａnd blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with CD31/PECAM-1 antibody [HEC7] in 3% BSA-PBS at a dilution of 1:100 ａnd incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST ａnd incubated with a proper secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin ａnd nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.