标题CD31 Antibody Catalog Number：OM274437

提供者：上海笃玛生物科技有限公司发布时间：2024/8/20 阅读次数：22次 >>进入该公司展台

Product Name	CD31 Antibody
Antibody Type	Primary Antibodies
Product description	CD31 is a cell adhesion molecule expressed on platelets ａnd at endothelial cell intercellular junctions.1) Simmons D.L., Walker C.J. Exp. Med. 171:2147-2152(1990)
Immunogen	This CD31 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 690-718 amino acids from the C-terminal region of human CD31.

Clonality	Polyclonal
Isotype	Ig
Host Species	Rabbit
Tested Applications	FACSIHC-PWB
	For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50:
Species Reactivity	Human
Concentration	1 mg/ml
Purification	Affinity purified

Gene Symbol	PECAM1
Alternative Names	Platelet endothelial cell adhesion molecule PECAM-1 EndoCAM GPIIA＇ PECA1 CD31 PECAM1 Hide
Molecular Weight(MW)	83 kDa

Entrez Gene	5175
Protein Accession	P16284

Application

Western Blot at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: human lung lysate Lane 3: THP-1 whole cell lysate Lysates/proteins at 20 ug per lane.
Application

Western blot analysis of CD31 antibody in HL60 cell line lysates (35ug/lane)
Application

Formalin-fixed ａnd paraffin-embedded human lung carcinoma with CD31 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application

Overlay histogram showing THP-1 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH1

Application Notes	For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50:

Form	Liquid
Storage Instructions	Store at 4˚C for three months ａnd -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.

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