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您的位置:首页 > 技术文献 > 产品说明 > TLR4 Catalog Number:OM628670

标题TLR4 Catalog Number:OM628670

   

提供者:上海笃玛生物科技有限公司    发布时间:2024/8/21   阅读次数:8次 >>进入该公司展台
 

Product Profile

Product Name TLR4
Antibody Type Primary Antibodies
Product description
Six human homologs of the Drosophila Toll receptor were initially identified based on their sequence similarities and designated toll-like receptors (TLR). Toll receptors are involved in mediating dorsoventral polarization in the developing Drosophila embryo and participate in the host immunity. The TLR family of proteins are characterized by a highly conserved Toll homology (TH) domain, which is essential for Toll-induced signal transduction. TLR1, as well as the other TLR family members, are type I transmembrane receptors that characteristically contain an extracellular domain consisting of several leucine-rich regions along with a single cylasmic Toll / IL-1R-like domain. TLR2 and TLR4 are activated in response to lipopolysacchride (LPS) stimulation, which results in the activation and translocation of NFkB and suggests that these receptors are involved in mediating inflammatory responses. Expression of TLR receptors is highest in peripheral blood leukocytes, macro-phages, and TLR6 is highly homologous to TLR1, sharing greater than 65% sequence identity, and, like other members of TLR family, it induces NFkB signaling upon activation.
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Immunogen
Peptide

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

ICCIHCFCWB

 
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
WB:1:500
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Species Reactivity

HumanMouseRat

Concentration 1 mg/mL.

Target Information

Alternative Names
ARMD10 antibody CD284 antibody CD284 antigen antibody Homolog of Drosophila toll antibody hToll antibody TLR 4 antibody TLR4 antibody TLR4_HUMAN antibody TOLL antibody Toll like receptor 4 antibody Toll-like receptor 4 antibody
Molecular Weight(MW) 96/91/73 kDa
Cellular Localization Cell membrane.

Database Links

SwissProt ID O00206
Q9QUK6
Q9QX05

Application

  • Application

    Fig1: ICC staining TLR4 in PMVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig2: ICC staining TLR4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig3: ICC staining TLR4 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig4: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-TLR4 antibody. Counter stained with hematoxylin.

  • Application

    Fig5: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-TLR4 antibody. Counter stained with hematoxylin.

  • Application

    Fig6: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-TLR4 antibody. Counter stained with hematoxylin.

  • Application

    Fig7: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-TLR4 antibody. Counter stained with hematoxylin.

  • Application

    Fig8: Flow cytometric analysis of Jurkat cells with TLR4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Positive Control Hela, HUVEC, PMVEC, rat kidney tissue, human skin tissue, human spleen tissue, mouse colon tissue, Jurkat.
Application Notes
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
WB:1:500
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Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.

关键词:稳定性强  特异性强    灵敏度高  

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