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Product Name | TLR4 Antibody (monoclonal) (M03) |
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Antibody Type | Primary Antibodies |
Clonality | Monoclonal |
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Isotype | IgG2a |
Clone Number | 1H7 |
Host Species | Mouse |
Tested Applications | WB |
WB~~1:500~1000: | |
Species Reactivity | Human |
Concentration | 1 mg/ml |
Purification |
Alternative Names | |
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Molecular Weight(MW) | 95680 Da |
Tissue Specificity | TLR4 (NP_612564, 214 a.a. ~ 292 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Entrez Gene | 7099 |
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Nucleotide Accession | NP_612564 |
Western Blot analysis of TLR4 expression in transfected 293T cell line by TLR4 monoclonal antibody (M03), clone 1H7.Lane 1: TLR4 transfected lysate(73.3 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of TLR4 over-expressed 293 cell line, cotransfected with TLR4 Validated Chimera RNAi ( Cat # H00007099-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TLR4 monoclonal antibody (M03), clone 1H7 (Cat # AT4256a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Application Notes | WB~~1:500~1000: |
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Form | Liquid |
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Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Storage Buffer | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
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