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| Product Name | Anti-PI3K p85 alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:200-1:1000 ICC:1:50-1:200 | |
| Species Reactivity | HumanNeisseria gonorrhoeaeRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PIK3R1 |
|---|---|
| Gene Synonyms | More |
| Gene Full Name | phosphoinositide-3-kinase regulatory subunit 1 |
| Gene Summary | Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit ａnd a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, ａnd a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011] Expression Ubiquitous expression in fat (RPKM 16.6), brain (RPKM 16.6) ａnd 24 other tissues See more More |
| Molecular Weight(MW) | 84kDa |
| Cellular Localization | Cylasmic |
Figure 1: Western blot analysis using PI3K p85 alpha antibody against Panc-1(1) cell lysate.
Figure2: Immunohistochemical analysis of paraffin-em
Figure 3: Immunofluorescence analysis of A549 cells using PI3K p85 alpha antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
| Application Notes | WB:1:500-1:2000 IHC:1:200-1:1000 ICC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300. |
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