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| Product Name | ZO-1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Tight junctions are complexes of proteins that create intercellular boundaries between the plasma membrane domains of epithelial ａnd endothelial cells. Many of the tight junction-associated proteins are members of the membrane-associated guanylate kinase (MAGUK) family ａnd include occludin, ZO-1, ZO-2 ａnd ZO-3 These proteins are thought to have both structural ａnd signaling roles, ａnd are characteristically defined by three protein-protein interaction modules: the PDZ domain, the SH3 domain ａnd the guanylate kinase (GuK) domain. ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin ａnd F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton ａnd the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 ａnd ZO-3 are ubiquitously expressed within epithelial tight junctions, ａnd unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in pithelial tissue. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| recommended for detection of ZO-1 of mouse, rat ａnd human origin by WB, IP, IF ａnd ELISA; also reactive with additional species, including equine, canine, bovine ａnd porcine: | |
| Species Reactivity | HumanMonkeyMouseRat |
| Concentration | 1 mg/ml |
| Purification | Affinity purified |
| Alternative Names | |
|---|---|
| Molecular Weight(MW) | 195KD |
| Tissue Specificity | epie corresponding to amino acids 1437-1736 mapping at the C-terminus of ZO-1 (Zona occludens-1) of human origin |
| Entrez Gene | 7082 |
|---|
Western blot analysis of ZO-1 ex
Western blot analysis of ZO-1 ex
Western blot analysis of ZO-1 ex
Western blot analysis of ZO-1 ex
| Application Notes | recommended for detection of ZO-1 of mouse, rat ａnd human origin by WB, IP, IF ａnd ELISA; also reactive with additional species, including equine, canine, bovine ａnd porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol. |
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