标题GCLM antibody Catalog Number：OM254278

提供者：上海笃玛生物科技有限公司发布时间：2024/8/22 阅读次数：20次 >>进入该公司展台

Product Name	GCLM antibody
Antibody Type	Primary Antibodies
Product description	Rabbit Polyclonal antibody to GCLM (glutamate-cysteine ligase, modifier subunit)Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit ａnd a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. [provided by RefSeq] More
Immunogen	Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human GCLM. The exact sequence is proprietary.

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Applications	ICC/IFIHC-PWB
	*Optimal dilutions/concentrations should be determined by the researcher. :
Species Reactivity	Human
Predicted Reactivity	Mouse, Rat, BovineMouse (100%), Rat (100%), Bovine (100%)
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration)
Purification	Affinity purified

Gene Synonyms	GLCLR antibody GCLM antibody GSC light chain antibody gamma-ECS regulatory subunit antibody GCS light chain antibody "glutamate-cysteine ligase (gamma-glutamylcysteine synthetase) regulatory (30.8kD) antibody" glutamate－－cysteine ligase modifier sub More
Molecular Weight(MW)	31 kDa (note) (2)

Application

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GCLM antibody at 1:200 dilution.
Application

Sample (30 µg of whole cell lysate) A: H1299 12% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application

Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using GCLM antibody at 1:500 dilution.
Application

Non-transfected (–) ａnd transfected (+) HeLa whole cell extracts (30 µg) were separated by 12% SDS-PAGE, ａnd the membrane was blotted with GCLM antibody [N1N3] diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Positive Control	HeLa , 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji
Application Notes	*Optimal dilutions/concentrations should be determined by the researcher. :

Form	Liquid
Storage Instructions	Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer	0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

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