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| Product Name | Rabbit anti-BRCA1 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The immunogen for anti-BRCA1 antibody: synthetic peptide directed towards the middle region of human BRCA1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1 mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BRCA1 |
|---|---|
| Gene Synonyms | More |
| Gene Full Name | Breast cancer 1, early onset |
| Gene Summary | The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination ａnd transcriptional regulation to maintain genomic stability. BRCA1 acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. BRCA1 plays a central role in DNA repair by facilitating cellular response to DNA repair. BRCA1 is required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase ａnd the G2 phase of the cell cycle. BRCA1 is involved in transcriptional regulation of P21 in response to DNA damage. BRCA1 is also required for FANCD2 targeting to sites of DNA damage.It may function as a transcriptional regulator. BRCA1 inhibits lipid synthesis by binding to inactive phosphorylated ACACA ａnd preventing its dephosphorylation.This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability ａnd acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, ａnd signal transducers to form a large multi-subunit protein complex known as BASC for BRCA1-associated genome surveillance complex. This gene product associates with RNA polymerase II, ａnd through the C-terminal domain, also interacts with histone deacetylase complex. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, ａnd recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers ａnd more than 80% of inherited breast ａnd ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization ａnd physiological function of this gene. Many alternatively spliced transcript variants have been described for this gene but only some have had their full-length natures identified. More |
| Alternative Names | More |
| Molecular Weight(MW) | 208kDa |
| Sequence | 1863 amino acids |
| Entrez Gene | 672 |
|---|---|
| SwissProt ID | P38398 |
| Protein Accession | NP_009225 |
Immunohistochemistry with Cell line 293T (Embyonic human kidney cells) tissue
0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Human Small Intestine
| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot ａnd store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol. |
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