标题Phospho-GATA6-Y271 Catalog Number：OM217336

提供者：上海笃玛生物科技有限公司发布时间：2024/8/27 阅读次数：23次 >>进入该公司展台

Product Name	Phospho-GATA6-Y271
Antibody Type	Primary Antibodies

Alternative Names	GATA6 Transcription factor GATA-6 GATA-binding factor 6
Molecular Weight(MW)	60033 Da
Function	Transcriptional activator that regulates SEMA3C ａnd PLXNA2. Thought to be important for regulating terminal differentiation and/or proliferation
Tissue Specificity	Expressed in heart, gut ａnd gut-derived tissues
Cellular Localization	Nucleus.

Entrez Gene	2627
Nucleotide Accession	NP_005248.2

Application

Fluorescent confocal image of HepG2 cells stained with phospho-GATA6-Y271 antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP3766a phospho-GATA6-Y271 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the phospho-GATA6 immunosignal mainly to the nucleus.
Application

Dot blot analysis of Phospho-GATA6-pY271 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.

Application Notes	IF~~1:100 DB~~1:100~500:

Form	Liquid
Storage Instructions	For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.

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