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Product Name | GRP78 antibody |
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Antibody Type | Primary Antibodies |
Product description |
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Immunogen | Synthetic peptide made to an internal portion of the human protein (within residues 250-300). [Swiss-Prot# P11021] |
Clonality | Polyclonal |
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Isotype | IgG |
Host Species | Rabbit |
Tested Applications | FACSICC/IFWB |
Recommended Starting Dilutions: For WB: Use at a concentration of 0.5 ug/ml. For FACS: Use at a dilution of 1:150. For ICC/IF: Use at a dilution of 1:50. Optimal dilutions/concentrations should be determined by the end user.: | |
Species Reactivity | HumanMouseChickenRatSheep |
Predicted Reactivity | Bovine, C. elegans, Drosophila, Primate, ZebrafishPrimate (100%), Bovine (100%), Zebrafish (100%), Drosophila (100%), C. elegans (100%) |
Concentration | 1 mg/ml |
Purification | Affinity purified |
Gene Synonyms | More |
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Target | HSPA5 |
Tissue Specificity | Grp78 |
Cellular Localization | Endoplasmic reticulum lumen. Melanosome. Note= Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
Simple Western: GRP78/HSPA5 Antibody [ - Simple Western lane view shows a specific band for GRP78 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Detection of Bip/Grp78 on HeLa whole cell extracts using
Application Notes | Recommended Starting Dilutions: For WB: Use at a concentration of 0.5 ug/ml. For FACS: Use at a dilution of 1:150. For ICC/IF: Use at a dilution of 1:50. Optimal dilutions/concentrations should be determined by the end user.: |
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Form | Liquid |
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Storage Instructions | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Storage Buffer | PBS, 30% glycerol and 0.1% sodium azide was added as a preservative. |
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