标题GRP78 antibody Catalog Number：OM255573

提供者：上海笃玛生物科技有限公司发布时间：2024/8/30 阅读次数：19次 >>进入该公司展台

Product Name	GRP78 antibody
Antibody Type	Primary Antibodies
Product description	Rabbit Polyclonal antibody to GRP78Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins ａnd degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate. More
Immunogen	Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse Hspa5. The exact sequence is proprietary.

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Applications	ICC/IFIHC-PWB
	*Optimal dilutions/concentrations should be determined by the researcher. :
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration)
Purification	Affinity purified

Gene Synonyms	GRP-78 antibody heat shock 70kD protein 5 (glucose-regulated protein 78kD) antibody glucose regulated protein 78 kDa antibody immunoglobulin heavy chain-binding protein antibody 78 kDa glucose-regulated protein antibody heat shock 70 kDa protein 5 a More
Molecular Weight(MW)	72 kDa (note) (2)
Tissue Specificity	Knockdown/Knockout validation was supported by customer review data.
Cellular Localization	Endoplasmic reticulum lumen , Melanosome

Application

Hspa5 antibody detects Hspa5 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Hspa5 protein stained by Hspa5 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application

Hspa5 antibody detects Hspa5 protein by Western blot analysis. A. 50 µg Rat heart lysate/extract B. 50 µg Rat Lung lysate/extract C. 50 µg Rat Liver lysate/extract D. 50 µg Rat kidney lysate/extract 7.5 % SDS-PAGE Hspa5 antibody dilution: 1:10000
Application

Hspa5 antibody detects Hspa5 protein at cytosol on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. Hspa5 antibody dilution: 1:500.
Application

Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 E: A375 7.5% SDS PAGE diluted at 1:10000
Application

Sample (30 ug of whole cell lysate) A: NIH-3T-3 B: JC C: BCL-1 D: C2C12 E: Raw264.7 7.5% SDS PAGE diluted at 1:10000
Application

Hspa5 antibody detects Hspa5 protein at cylasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Hspa5 antibody diluted at 1:500.
Application

Hspa5 antibody detects Hspa5 protein at cylasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Hspa5 antibody diluted at 1:500.

Positive Control	293T , A431 , HeLa , HepG2 , A375 , NIH-3T-3 , JC , BCL-1 , C2C12 , Raw264.7 , rat heart , rat lung , rat liver , rat kidney
Application Notes	*Optimal dilutions/concentrations should be determined by the researcher. :

Form	Liquid
Storage Instructions	Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer	1XPBS, 0.05% Sodium Azide was added as a preservative.

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