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您的位置:首页 > 技术文献 > 产品说明 > GUCY1A3 [B2-G6] Catalog Number:OM627610

标题GUCY1A3 [B2-G6] Catalog Number:OM627610

   

提供者:上海笃玛生物科技有限公司    发布时间:2024/9/2   阅读次数:23次 >>进入该公司展台
 

Product Profile

Product Name GUCY1A3 [B2-G6]
Antibody Type Primary Antibodies
Product description
Guanylate cyclases belong to the adenylyl cyclase class-4/guanylyl cyclase family. There are two forms of guanylate cyclase. The soluble forms, known as GCS or sGC, act as receptors for nitric oxide. The membrane-bound receptor forms, known as GC, are peptide hormone receptors. GCS, a cGMP-synthesizing enzyme, is the major receptor for the neurotransmitter nitric oxide (NO). It plays a crucial role in smooth muscle contractility, platelet reactivity and neurotransmission. GCS is a heme containing heterodimer, consisting of one α subunit, designated GCS-α-1, and one β subunit. The heme moeity mediates NO activation, and this heme group also binds carbon monoxide, which weakly stimulates the enzyme. Both NO and CO stimulation are enhanced by the allosteric activator 3-(5'-hydroxymethyl-2'furyl)-benzyl -indazole, YC-1. YC-1 can also stimulate GCS in a NO-independent manner. Both the α and β subunits are required for cGMP generation, and at least two isoforms exist for each subunit. Heterodimers consisting of α-1/β-1 and α-2/β-1 have been identified, and both display similar enzymatic activity.
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Immunogen
Recombinant protein

Key Feature

Clonality Monoclonal
Isotype IgG1
Host Species Mouse
Tested Applications

WBICCIHCFC

 
WB:1:250~1:1000
ICC:1:100~1:200
IHC:1:20~1:100
FC:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
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Species Reactivity

Human

Concentration 2 mg/mL.

Target Information

Alternative Names
GC S alpha 1 antibody GC SA3 antibody GCS alpha 1 antibody GCS alpha 3 antibody GCS-alpha-1 antibody GCS-alpha-3 antibody GCYA3_HUMAN antibody Guanylate cyclase 1 soluble alpha 3 antibody Guanylate cyclase soluble subunit alpha 1 antibody Guanylate cyclase soluble subunit alpha 3 antibody Guanylate cyclase soluble subunit alpha-3 antibody Guanylate cyclase
nitric oxide-sensitive
alpha-1 subunit antibody Guc1a1 antibody GUC1A3 antibody GUCA3 antibody GUCSA3 antibody Gucy1a1 antibody Gucy1a3 antibody MYMY6 antibody nitric oxide-sensitive Guanylate cyclase
alpha-1 subunit antibody NOGC
alpha-1 subunit antibody Soluble guanylate cyclase large subunit antibody
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Molecular Weight(MW) 77 kDa
Cellular Localization Cylasm.

Database Links

SwissProt ID Q02108

Application

  • Application

    Fig1: Western blot analysis of GUCY1A3 on HEK293 (1) and GUCY1A3-hIgGFc transfected HEK293 (2) cell lysate using anti-GUCY1A3 antibody at 1/1,000

  • Application

    Fig2: Western blot analysis of GUCY1A3 on different cell lysate using anti-GUCY1A3 antibody at 1/1,000 dilution. Positive control: Line1:HEK293        Line2:Raji

  • Application

    Fig3: ICC staining GUCY1A3 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig4: Immunohistochemical analysis of paraffin-embedded human renal cancer tissue using anti-GUCY1A3 antibody. Counter stained with hematoxylin.

  • Application

    Fig5: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-GUCY1A3 antibody. Counter stained with hematoxylin.

  • Application

    Fig6: Flow cytometric analysis of HEK293 cells with GUCY1A3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).

Positive Control GUCY1A3-hIgGFc transfected HEK293 cell lysate, HEK293, Raji cell lysate, HepG2, human renal cancer tissue, human esophageal cancer tissue, HEK293.
Application Notes
WB:1:250~1:1000
ICC:1:100~1:200
IHC:1:20~1:100
FC:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
More 

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

关键词:稳定性强  特异性强    灵敏度高  

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