标题DKK3 Antibody Catalog Number：OM277084

提供者：上海笃玛生物科技有限公司发布时间：2024/9/4 阅读次数：16次 >>进入该公司展台

Product Name	DKK3 Antibody
Antibody Type	Primary Antibodies
Product description	DKK3, like DKK1, DKK2, ａnd DKK4, possesses an N-terminal signal peptide ａnd 2 conserved cysteine-rich domains, which are separated by a linker region ａnd contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, ａnd DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis revealed wide expression of the DKK3 transcript, with highest expression in heart, brain, ａnd spinal cord. In situ hybridization reveals highest expression in mouse brain, eye, ａnd heart.1) Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003). More
Immunogen	This DKK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-45 amino acids from the N-terminal region of human DKK3.

Clonality	Polyclonal
Isotype	Ig
Host Species	Rabbit
Tested Applications	IHC-PWB
	For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100:
Species Reactivity	Human
Concentration	1 mg/ml
Purification	Affinity purified

Gene Symbol	DKK3
Alternative Names	Dickkopf-related protein 3 Dickkopf-3 Dkk-3 hDkk-3 DKK3 REIC More
Molecular Weight(MW)	38 kDa

Entrez Gene	27122
Protein Accession	Q9UBP4

Application

Formalin-fixed ａnd paraffin-embedded human breast carcinoma tissue reacted with DKK3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application

Formalin-fixed ａnd paraffin-embedded human brain tissue reacted with DKK3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application

Western blot analysis of lysates from 293, 293T, A549, HL-60, MCF-7 cell line ａnd mouse brain tissue lysate (from left to right), using DKK3 Antibody (A30) at 1:1000 at each lane.

Application Notes	For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100:

Form	Liquid
Storage Instructions	Store at 4˚C for three months ａnd -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.

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