标题CD55 Antibody (N-term) Catalog Number：OM229252

提供者：上海笃玛生物科技有限公司发布时间：2024/9/5 阅读次数：15次 >>进入该公司展台

Product Name	CD55 Antibody (N-term)
Antibody Type	Primary Antibodies

Gene Synonyms	CR DAF
Alternative Names	CD55 CR DAF Complement decay-accelerating factor CD_antigen=CD55 Flags: Precursor More
Molecular Weight(MW)	41400 Da
Function	This protein recognizes C4b ａnd C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b ａnd C3b are locally generated during C4 ａnd c3 activation Interaction of daf with cell-associated C4b ａnd C3b polypeptides interferes with their ability to catalyze the conversion of C2 ａnd factor B to enzymatically active C2a ａnd Bb ａnd thereby prevents the formation of C4b2a ａnd C3bBb, the amplification convertases of the complement cascade More
Tissue Specificity	Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, ａnd variants of the molecule are present in body fluids ａnd in extracellular matrix
Cellular Localization	Isoform 1: Cell membrane; Single-pass type I membrane protein

Entrez Gene	1604
Nucleotide Accession	NP_000565.1;NP_001108224.1

Application

CD55 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CD55 antibody detected the CD55 protein (arrow).
Application

Confocal immunofluorescent analysis of CD55 Antibody (N-term)(Cat#AP14798a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application

CD55 Antibody (N-term) immunohistochemistry analysis in formalin fixed ａnd paraffin embedded human lung adenocarcinoma followed by peroxidase conjugation of the secondary antibody ａnd DAB staining.This data demonstrates the use of CD55 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application

CD55 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Application Notes	WB~~1:100~500 IF~~1:10~50 IHC~~1:10~50 FC~~1:10~50:

Form	Liquid
Storage Instructions	For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

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