标题MARK3 [H12-G2] Catalog Number：OM627647

提供者：上海笃玛生物科技有限公司发布时间：2024/9/5 阅读次数：22次 >>进入该公司展台

Product Name	MARK3 [H12-G2]
Antibody Type	Primary Antibodies
Product description	MARK3 (MAP/microtubule affinity-regulating kinase 3), also known as C-TAK1 (Cdc25C-associated protein kinase 1), EMK-2 (ELKL motif kinase 2), protein kinase STK10 or serine/threonine-protein kinase p78, is a 753 amino acid peripheral membrane protein that phosphorylates Cdc25C on Serine 216 ａnd is ubiquitously expressed in various human tissue ａnd cell lines. Existing as six alternatively spliced isoforms, MARK3 belongs to the Ser/Thr protein kinase family ａnd the protein kinase superfamily. MARK3 has been suggested to mediate the binding of the 14-3-3 proteins through its kinase activity ａnd acts as a negative regulator of mitosis. The gene encoding MARK3 maps to human chromosome 14q32.3 ａnd mouse chromosome 12 F1. More
Immunogen	Recombinant protein

Clonality	Monoclonal
Isotype	IgG1
Host Species	Mouse
Tested Applications	WBIHCFC
	WB:1:250~1:1000 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. More
Species Reactivity	HumanMouse
Concentration	2 mg/mL.

Alternative Names	C-TAK1 antibody Cdc25C associated protein kinase 1 antibody Cdc25C-associated protein kinase 1 antibody cTAK1 antibody ELKL motif kinase 2 antibody EMK-2 antibody Emk2 antibody ETK 1 antibody KIAA4230 antibody KP78 antibody MAP microtubule affinity regulating kinase 3 antibody MAP/microtubule affinity-regulating kinase 3 antibody Mark3 antibody MARK3_HUMAN antibody Par 1a antibody PAR1A antibody Protein kinase STK10 antibody Ser/Thr protein kinase PAR-1 antibody Serine threonine protein kinase p78 antibody Serine/threonine-protein kinase p78 antibody SerThr protein kinase PAR 1 antibody More
Molecular Weight(MW)	84 kDa
Cellular Localization	Cell membrane. Peripheral membrane protein.

SwissProt ID	P27448

Application

Fig1: Western blot analysis of MARK3 on human MARK3 recombinant protein using anti-MARK3 antibody at 1/1,000 dilution.
Application

Fig2: Western blot analysis of MARK3 on different cell lysate using anti-MARK3 antibody at 1/1,000 dilution. Positive control：Line1:HeLaLine2:SK-N-SHLine3:K562Line4:HCT116Line5:HEK293Line6:3T3L1Line7:NIH3T3Line8:JurkatLine9:A431
Application

Fig3: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-MARK3 antibody. Counter stained with hematoxylin.
Application

Fig4: Flow cytometric analysis of SK-N-SH cells with MARK3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

Positive Control	Human MARK3 recombinant protein, HeLa, SK-N-SH, K562, HCT116, HEK293, 3T3L1, NIH3T3, Jurkat, ａnd A431 cell lysate, human bladder cancer tissue, SK-N-SH.
Application Notes	WB:1:250~1:1000 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. More

Form	Liquid
Storage Instructions	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer	1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.

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