标题Annexin A1 antibody Catalog Number：OM239514

提供者：上海笃玛生物科技有限公司发布时间：2024/9/9 阅读次数：7次 >>进入该公司展台

Product Name	Annexin A1 antibody
Antibody Type	Primary Antibodies
Product description	Rabbit Polyclonal antibody to Annexin A1 (annexin A1)Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 ａnd are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins ａnd leukotrienes, annexin I may have potential anti-inflammatory activity. [provided by RefSeq] More
Immunogen	Recombinant protein encompassing a sequence within the center region of human Annexin A1. The exact sequence is proprietary.

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Applications	ICC/IFIHC-PIPWB
	*Optimal dilutions/concentrations should be determined by the researcher. :
Species Reactivity	HumanMouseRat
Predicted Reactivity	Dog, Pig, Rabbit, Chimpanzee, BovineDog (91%), Pig (88%), Rabbit (91%), Chimpanzee (100%), Bovine (90%)
Concentration	1 mg/ml
Purification	Affinity purified

Gene Synonyms	ANX1 antibody LPC1 antibody ANXA1 antibody calpactin-2 antibody chromobindin-9 antibody annexin-1 antibody phospholipase A2 inhibitory protein antibody calpactin II antibody lipocortin I antibody annexin I (lipocortin I) antibody annexin A1 antib More
Molecular Weight(MW)	39 kDa (note)
Cellular Localization	Nucleus , Cylasm , Cell projection , cilium , Basolateral cell membrane

Application

Immunoprecipitation of Annexin A1 protein from HeLa whole cell extracts using 5 μg of Annexin A1 antibody . Western blot analysis was performed using Annexin A1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Annexin I antibody at 1:200 dilution.
Application

Immunohistochemical analysis of paraffin-embedded N87 Xenograft , using Annexin I antibody at 1:500 dilution.
Application

Sample (30 µg of whole cell lysate) A: A431 B: H1299 C: HeLa 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application

Sample (30 µg of whole cell lysate) A: NIH-3T3 B: JC 10% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application

Wild-type (WT) ａnd Annexin A1 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, ａnd the membrane was blotted with Annexin A1 antibody diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application

Annexin A1 antibody detects Annexin A1 protein at cytosol ａnd nucleus on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. Annexin A1 antibody dilution: 1:500.

Positive Control	A431 , H1299 , HeLa , Molt-4 , NIH-3T3 , JC
Application Notes	*Optimal dilutions/concentrations should be determined by the researcher. :

Form	Liquid
Storage Instructions	Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer	0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

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