标题SPIB Catalog Number：OM640801

提供者：上海笃玛生物科技有限公司发布时间：2024/9/9 阅读次数：19次 >>进入该公司展台

Product Name	SPIB
Antibody Type	Primary Antibodies
Immunogen	Purified recombinant fragment of human SPIB (AA: 200-252) expressed in E. Coli.

Clonality	Monoclonal
Host Species	Unknow
Tested Applications	WBIHCICCFCMICC/IF
	WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. More
Species Reactivity	HumanMouse

Alternative Names	SPI-B
Molecular Weight(MW)	28.8kDa

Entrez Gene	6689

Application

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application

Figure 1: Western blot analysis using SPIB mAb against human SPIB recombinant protein. (Expected MW is 32 kDa)
Application

Figure 2: Western blot analysis using SPIB mouse mAb against A549 (1), PC-3 (2), ａnd NIH3T3 (3) cell lysate.
Application

Figure 3: Immunofluorescence analysis of HeLa cells using SPIB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Application

Figure 4: Flow cytometric analysis of NIH3T3 cells using SPIB mouse mAb (green) ａnd negative control (red).
Application

Figure 5: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using SPIB mouse mAb with DAB staining.
Application

Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal cancerwith DAB staining.

Application Notes	WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. More

Form	Ascitic fluid containing 0.03% sodium azide.
Storage Instructions	4℃; -20℃ for long term storage

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