标题ARHGDIA antibody Catalog Number：OM240098

提供者：上海笃玛生物科技有限公司发布时间：2024/9/10 阅读次数：12次 >>进入该公司展台

Product Name	ARHGDIA antibody
Antibody Type	Primary Antibodies
Product description	Mouse monoclonal antibody [1F2] to ARHGDIARegulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, ａnd the subsequent binding of GTP to them.
Immunogen	Full length human recombinant protein of human ARHGDIA (NP_004300) produced in HEK293T cell.

Gene Synonyms	MGC117248 GDIA1 RHOGDI ARHGDIA More
Target	ARHGDIA
Tissue Specificity	is specific for human ARHGDIA (NP_004300).

Application

HEK293T cells transfected with either RC200902 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ARHGDIA antibody, ａnd then analyzed by flow cytometry.
Application

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARHGDIA (Right lane) cDNA for 48 hrs ａnd lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE ａnd immunoblotted with anti-ARHGDIA.
Application

Flow cytometric Analysis of Jurkat cells, using anti-ARHGDIA antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Application

Flow cytometric Analysis of Hela cells, using anti-ARHGDIA antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Application

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARHGDIA monoclonal antibody.

Application Notes	IHC 1:50 :

Form	Liquid
Storage Instructions	For short-term storage, store at 4°C or aliquot into working amounts ａnd store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles.
Storage Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol ａnd 0.02% sodium azide.

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