标题ARHGDIA Antibody (C-term) Catalog Number：OM226825

提供者：上海笃玛生物科技有限公司发布时间：2024/9/10 阅读次数：17次 >>进入该公司展台

Product Name	ARHGDIA Antibody (C-term)
Antibody Type	Primary Antibodies

Gene Synonyms	GDIA1
Alternative Names	ARHGDIA GDIA1 Rho GDP-dissociation inhibitor 1 Rho-GDI alpha More
Molecular Weight(MW)	23207 Da
Function	Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, ａnd the subsequent binding of GTP to them. In glioma cells, inhibits cell migration ａnd invasion by mediating the signals of SEMA5A ａnd PLXNB3 that lead to inactivation of RAC1 (By similarity) More
Tissue Specificity	This ARHGDIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119~148 amino acids from the C-terminal region of human ARHGDIA.
Cellular Localization	Cylasm.

Entrez Gene	396

Application

Western blot analysis of ARHGDIA Antibody (C-term) in A375(lane 1),HL-60(lane 2),Ramos(lane 3) cell line lysates (35ug/lane). ARHGDIA (arrow) was detected using the purified Pab.
Application

Western blot analysis of ARHGDIA (arrow) using rabbit polyclonal ARHGDIA Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ARHGDIA gene.
Application

Formalin-fixed ａnd paraffin-embedded human brain tissue reacted with ARHGDIA Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application

ARHGDIA Antibody (C-term) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell ( histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Application Notes	WB~~1:100~500 IHC~~1:10~50 FC~~1:10~50:

Form	Liquid
Storage Instructions	For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

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