标题PPM1A antibody Catalog Number：OM269347

提供者：上海笃玛生物科技有限公司发布时间：2024/9/11 阅读次数：18次 >>进入该公司展台

Product Name	PPM1A antibody
Antibody Type	Primary Antibodies
Product description	Rabbit Polyclonal antibody to PPM1A (protein phosphatase, Mg2+/Mn2+ dependent, 1A)The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, ａnd negatively regulates the activities of, MAP kinases ａnd MAP kinase kinases. It has been shown to inhibit the activation of p38 ａnd JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, ａnd thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest ａnd apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq] More
Immunogen	Recombinant protein encompassing a sequence within the center region of human PPM1A. The exact sequence is proprietary.

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Applications	ICC/IFIHC-PWB
	*Optimal dilutions/concentrations should be determined by the researcher. :
Species Reactivity	HumanMouseRat
Predicted Reactivity	Xenopus laevis, Rabbit, BovineXenopus laevis (89%), Rabbit (99%), Bovine (99%)
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration)
Purification	Affinity purified

Gene Synonyms	FLJ42306 antibody MGC9201 antibody PP2C-ALPHA antibody PP2CA antibody PP2Calpha antibody PPM1A antibody protein phosphatase 1A (formerly 2C) magnesium-dependent alpha isoform antibody protein phosphatase 1A antibody protein phosphatase Mg2+/Mn2+ More
Molecular Weight(MW)	42 kDa (note) (3)

Application

Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000
Application

Sample (30 ug of whole cell lysate) A: 293T B: A431 10% SDS PAGE diluted at 1:1000
Application

PPM1A antibody detects PPM1A protein at cylasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: PPM1A protein stained by PPM1A antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PPM1A antibody at 1:500 dilution.
Application

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, ａnd the membrane was blotted with PPM1A antibody diluted at 1:500.

Positive Control	293T , A431 , mouse brain , PC-12 , Rat2
Application Notes	*Optimal dilutions/concentrations should be determined by the researcher. :

Form	Liquid
Storage Instructions	Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer	0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

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