标题MELK antibody Catalog Number：OM263000

提供者：上海笃玛生物科技有限公司发布时间：2024/9/12 阅读次数：18次 >>进入该公司展台

Product Name	MELK antibody
Antibody Type	Primary Antibodies
Product description	Rabbit Polyclonal antibody to MELK (maternal embryonic leucine zipper kinase)
Immunogen	Recombinant protein encompassing a sequence within the center region of human MELK. The exact sequence is proprietary.

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Applications	ICC/IFIHC-PWB
	*Optimal dilutions/concentrations should be determined by the researcher. :
Species Reactivity	HumanMouse
Predicted Reactivity	Rat, Xenopus laevis, Chicken, BovineRat (96%), Xenopus laevis (80%), Chicken (80%), Bovine (95%)
Concentration	1 mg/ml
Purification	Affinity purified

Gene Synonyms	HPK38 antibody KIAA0175 antibody MELK antibody pEg3 kinase antibody protein kinase Eg3 antibody maternal embryonic leucine zipper kinase antibody hMELK antibody tyrosine-protein kinase MELK antibody protein kinase PK38 antibody More
Molecular Weight(MW)	75 kDa (note) (2)
Cellular Localization	Cylasm

Application

Sample (30 µg of whole cell lysate) A: HeLa B: HCT116 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application

MELK antibody detects MELK protein at cylasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. MELK antibody diluted at 1:500.
Application

MELK antibody detects MELK protein at cylasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. MELK antibody diluted at 1:500.
Application

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using MELK antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.

Positive Control	A549 , HeLa , HepG2 , HCT116
Application Notes	*Optimal dilutions/concentrations should be determined by the researcher. :

Form	Liquid
Storage Instructions	Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer	0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

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