标题MELK [B7-G2] Catalog Number：OM627794

提供者：上海笃玛生物科技有限公司发布时间：2024/9/12 阅读次数：12次 >>进入该公司展台

Product Name	MELK [B7-G2]
Antibody Type	Primary Antibodies
Product description	Maternal embryonic leucine zipper kinase (KIAA0175, HPK38) or MELK, a new member of the Snf1/AMPK family of kinases, encodes a protein with a kinase catalytic domain ａnd a leucine zipper motif consisting of a periodic repetition of leucine residues at every seventh residue located within the N-terminal catalytic domain. This motif has been observed in myriad DNA-binding proteins ａnd is presumed to be involved in protein-DNA interactions, ａnd potentially protein-protein interactions. Research predicts that the gene product of MELK plays a role in the signal transduction events in the egg ａnd early embryo. Mouse ａnd human MELK proteins share 95% sequence identity in the kinase domain ａnd northern blot analysis in mouse indicates that MELK expression is restricted to spermatogonia in the testis ａnd to oocytes in the ovary. More
Immunogen	Recombinant protein

Alternative Names	AI327312 antibody hMELK antibody HPK 38 antibody hPK38 antibody KIAA0175 antibody Likely ortholog of maternal embryonic leucine zipper kinase antibody Maternal embryonic leucine zipper kinase antibody MELK antibody MELK_HUMAN antibody mKIAA0175 antibody MPK38 antibody OTTHUMP00000021377 antibody OTTHUMP00000046113 antibody pEg3 kinase antibody Protein kinase Eg3 antibody Protein kinase PK38 antibody RP23 382O11.1 antibody Tyrosine protein kinase MELK antibody More
Molecular Weight(MW)	75kDa
Cellular Localization	Cell membrane.

SwissProt ID	Q14680

Application

Fig1: ICC staining MELK (green) ａnd actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application

Fig2: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-MELK antibody. Counter stained with hematoxylin.
Application

Fig3: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-MELK antibody. Counter stained with hematoxylin.
Application

Fig4: Flow cytometric analysis of MCF-7 cells with MELK antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

Positive Control	HepG2, human liver cancer tissue, human rectum cancer tissue, MCF-7.
Application Notes	ICC:1:200-1:1000 IHC:1:200-1:1,000 FC:1:100-1:200

Form	Liquid
Storage Instructions	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer	1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.

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