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| Product Name | MELK Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB~~1:100~500: | |
| Species Reactivity | Human |
| Concentration | 1 mg/ml |
| Purification |
| Gene Synonyms | |
|---|---|
| Alternative Names | More |
| Molecular Weight(MW) | 74642 Da |
| Function | Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis ａnd splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 ａnd ZNF622. Acts as an activator of apoptosis by phosphorylating ａnd activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome ａnd the spindle poles during mitosis Plays a key role in cell proliferation ａnd carcinogenesis Required for proliferation of embryonic ａnd postnatal multipotent neural progenitors. Phosphorylates ａnd inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hemaoiesis More |
| Tissue Specificity | Expressed in placenta, kidney, thymus, testis, ovary ａnd intestine |
| Cellular Localization | Cell membrane; Peripheral membrane protein. |
| Entrez Gene | 9833 |
|---|
The anti-MELK Pab is used in Western blot to detect MKEL in HEPG2 tissue lysate
| Application Notes | WB~~1:100~500: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high ａnd low pH buffers ａnd neutralized immediately, followed by dialysis against PBS. |
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