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| Product Name | MAPK8 Antibody (T183/Y185) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB~~1:100~500 IHC~~1:50~100: | |
| Species Reactivity | Human |
| Concentration | 1 mg/ml |
| Purification |
| Gene Synonyms | More |
|---|---|
| Alternative Names | More |
| Molecular Weight(MW) | 48296 Da |
| Function | Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation ａnd programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 ａnd MAP2K7/MKK7 phosphorylate ａnd activate MAPK8/JNK1 In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 ａnd ATF2 ａnd thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 ａnd the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 ａnd Yes-associates protein YAP1. In T-cells, MAPK8 ａnd MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, ａnd thus activation of auhagy Phosphorylates STMN2 ａnd hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cylasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage ａnd of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH More |
| Tissue Specificity | This JNK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 162-199 amino acids from human JNK1. |
| Cellular Localization | Cylasm. Nucleus. |
| Entrez Gene | 5599 |
|---|---|
| Nucleotide Accession | NP_620637 |
JNK1-T183/Y185 Antibody western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the JNK1 antibody detected the JNK1 protein (arrow).
JNK1-T183/Y185 antibody immunohistochemistry analysis in formalin fixed ａnd paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody ａnd DAB staining. This data demonstrates the use of the LJNK1-T183/Y185 antibody for immunohistochemistry. Clinical relevance has not been evaluated.
| Application Notes | WB~~1:100~500 IHC~~1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
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