标题EIF4B Antibody Catalog Number：OM277791

提供者：上海笃玛生物科技有限公司发布时间：2024/9/13 阅读次数：1次 >>进入该公司展台

Product Name	EIF4B Antibody
Antibody Type	Primary Antibodies
Product description	EIF4B is required for the binding of mRNA to ribosomes. It functions in close association with EIF4-F ａnd EIF4-A by binding near the 5＇- terminal cap of mRNA in presence of EIF-4F ａnd ATP. This gene also promotes the ATPase activity ａnd the ATP-dependent RNA unwinding activity of both EIF4-A ａnd EIF4-F1) Doepker, R.C., et al., J. Virol. 78(9):4684-4699 (2004). More
Immunogen	This EIF4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 546-575 amino acids from the C-terminal region of human EIF4B.

Clonality	Polyclonal
Isotype	Ig
Host Species	Rabbit
Tested Applications	IHC-PWB
	For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100:
Species Reactivity	Human
Concentration	1 mg/ml

Gene Symbol	EIF4B
Alternative Names	Eukaryotic translation initiation factor 4B eIF-4B EIF4B
Molecular Weight(MW)	69 kDa
Sequence Similarities	Predicted species reactivity based on immunogen sequence: Mouse

Entrez Gene	1975
Protein Accession	P23588

Application

Formalin-fixed ａnd paraffin-embedded human breast carcinoma reacted with EIF4B Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application

Western blot analysis of anti-EIF4B Antibody Pab in 293 cell line lysates transiently transfected with the EIF4B gene (2ug/lane).
Application

Western blot analysis of anti-EIF4B Antibody Pab in K562 cell line lysates (35ug/lane).
Application

Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.

Application Notes	For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100:

Form	Liquid
Storage Instructions	Store at 4˚C for three months ａnd -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.

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