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您的位置:首页 > 技术文献 > 产品说明 > eIF4B antibody Catalog Number:OM251212

标题eIF4B antibody Catalog Number:OM251212

   

提供者:上海笃玛生物科技有限公司    发布时间:2024/9/13   阅读次数:12次 >>进入该公司展台
 

Product Profile

Product Name eIF4B antibody
Antibody Type Primary Antibodies
Product description
Rabbit Polyclonal antibody to eIF4BRequired for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
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Immunogen
Peptide sequence around aa.420~424 (T-G-S-E-S) derived from Human eIF4B.

Key Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

IHC-PWB

 
Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:
Species Reactivity

Human

Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification Affinity purified

Target Information

Gene Synonyms
EIF-4B
PRO1843
EIF4B
eukaryotic translation initiation factor 4B
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Target eIF4B
Molecular Weight(MW) 80 (note)

Application

  • Application

    Western blot analysis of extracts from 3T3 and HL60 cells using eIF4B(Ab-422) Antibody

  • Application

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using eIF4B Antibody (left) or the same antibody preincubated with blocking peptide (right).

Application Notes
Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:

Additional Information

Form Liquid
Storage Instructions Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

关键词:稳定性强  特异性强    灵敏度高  

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