标题EIF4B [D7-F5] Catalog Number：OM627752

提供者：上海笃玛生物科技有限公司发布时间：2024/9/13 阅读次数：13次 >>进入该公司展台

Product Name	EIF4B [D7-F5]
Antibody Type	Primary Antibodies
Product description	The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors ａnd RNA molecules. These interactions are facilitated, in part, by the eukaryotic initiation factor 4 family (eIF4) of proteins that are involved in the early initiation of protein synthesis. eIF4B (eukaryotic translation initiation factor 4B) is a 611 amino acid protein that contains one RNA recognition motif ａnd belongs to the eIF4 protein family. Required for proper mRNA/ribosome binding, eIF4B associates with other eIF4 proteins, such as eIF4A, ａnd promotes the ATP-dependent unwinding activity of ｓelect eukaryotic initiation factors. The gene encoding eIF4B maps to human chromosome 12, which encodes over 1,100 genes ａnd comprises approximately 4.5% of the human genome. More
Immunogen	Recombinant protein

Alternative Names	2310046H11Rik antibody AL024095 antibody C85189 antibody EIF 4B antibody eIF-4B antibody EIF4B antibody Eukaryotic initiation factor 4B antibody Eukaryotic translation initiation factor 4B antibody IF4B_HUMAN antibody PRO1843 antibody
Molecular Weight(MW)	80 kDa
Cellular Localization	Cytosol.

SwissProt ID	P23588 Q8BGD9

Application

Fig1: Western blot analysis of EIF4B on human EIF4B recombinant protein using anti-EIF4B antibody at 1/1,000 dilution.
Application
Application

Fig3: ICC staining EIF4B (green) ａnd actin filaments (red) in NIH-3T3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application

Fig4: Immunohistochemical analysis of paraffin-embedded colon cancer tissue using anti-EIF4B antibody. Counter stained with hematoxylin.
Application

Fig5: Flow cytometric analysis of Hela cells with EIF4B antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

Positive Control	Human EIF4B recombinant protein, A431, A549, HepG2, HEK293, HeLa, Jurkat, K562, NIH-3T3, MCF-7, colon cancer tissue.
Application Notes	WB:1:500-1:2,000 ICC:1:100-1:500 IHC:1:100-1:500 FC:1:100-1:200 More

Form	Liquid
Storage Instructions	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer	1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.

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