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| Product Name | EIF4B Antibody (Center S498) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB~~1:50~100 IHC~~1:50~100 FC~~1:10~50: | |
| Species Reactivity | Human |
| Concentration | 1 mg/ml |
| Purification |
| Alternative Names | |
|---|---|
| Molecular Weight(MW) | 69151 Da |
| Function | Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F ａnd EIF4-A. Binds near the 5＇- terminal cap of mRNA in presence of EIF-4F ａnd ATP. Promotes the ATPase activity ａnd the ATP-dependent RNA unwinding activity of both EIF4-A ａnd EIF4-F |
| Tissue Specificity | This EIF4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Central region of human EIF4B. |
| Entrez Gene | 1975 |
|---|---|
| Nucleotide Accession | NP_001408.2 |
EIF4B Antibody (pS498) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the EIF4B antibody detected the EIF4B protein (arrow).
Western blot analysis of EIF4B (arrow) using rabbit polyclonal EIF4B Antibody (pS498) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4B gene.
EIF4B Antibody (Center S498) immunohistochemistry analysis in formalin fixed ａnd paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody ａnd DAB staining.This data demonstrates the use of EIF4B Antibody (Center S498) for immunohistochemistry. Clinical relevance has not been evaluated.
EIF4B Antibody (Center S498) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
| Application Notes | WB~~1:50~100 IHC~~1:50~100 FC~~1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
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