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Product Name | EIF4B Antibody (C-term) |
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Antibody Type | Primary Antibodies |
Clonality | Polyclonal |
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Isotype | Ig |
Host Species | Rabbit |
Tested Applications | WBIHC |
WB~~1:50~100 IHC~~1:50-100: | |
Species Reactivity | Human |
Concentration | 1 mg/ml |
Purification |
Alternative Names | |
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Molecular Weight(MW) | 69151 Da |
Function | Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F |
Tissue Specificity | This EIF4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 553~583 amino acids from the C-terminal region of human EIF4B Antibody. |
Entrez Gene | 1975 |
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Nucleotide Accession | Q8WYK5 |
Western blot analysis of anti-EIF4B Antibody (C-term) Pab in K562 cell line lysates (35ug/lane). EIF4B(arrow) was detected using the purified Pab (1:60 dilution).
Western blot analysis of anti-EIF4B Antibody (C-term) Pab in 293 cell line lysates transiently transfected with the EIF4B gene (2ug/lane). EIF4B(arrow) was detected using the purified Pab (1:60 dilution).
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EIF4B Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application Notes | WB~~1:50~100 IHC~~1:50-100: |
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Form | Liquid |
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Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
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