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您的位置:首页 > 技术文献 > 产品说明 > EIF4B Antibody Catalog Number:OM232037

标题EIF4B Antibody Catalog Number:OM232037

   

提供者:上海笃玛生物科技有限公司    发布时间:2024/9/13   阅读次数:14次 >>进入该公司展台
 

Product Profile

Product Name EIF4B Antibody
Antibody Type Primary Antibodies

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

WBIHCFC

 
WB~~1:100~500 IHC~~1:50~100 FC~~1:10~50:
Species Reactivity

Human

Concentration 1 mg/ml
Purification  

Target Information

Alternative Names
EIF4B
Eukaryotic translation initiation factor 4B
Molecular Weight(MW) 69151 Da
Function
Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F
Tissue Specificity This EIF4B antibody is generated from rabbits immunized with human EIF4B recombinant protein.

Database Links

Entrez Gene 1975
Nucleotide Accession Q8WYK5

Application

  • Application

    Western blot analysis of EIF4B Antibody in NCI-H460 cell line lysates (35ug/lane). EIF4B (arrow) was detected using the purified Pab.

  • Application

    Formalin-fixed and paraffin-embedded human testis tissue reacted with EIF4B Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clini relevance has not been evaluated.

  • Application

    EIF4B Antibody flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Application Notes
WB~~1:100~500 IHC~~1:50~100 FC~~1:10~50:

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

关键词:稳定性强  特异性强    灵敏度高  

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