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Product Name | EIF4B Antibody |
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Antibody Type | Primary Antibodies |
Clonality | Polyclonal |
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Isotype | Ig |
Host Species | Rabbit |
Tested Applications | WBIHCFC |
WB~~1:100~500 IHC~~1:50~100 FC~~1:10~50: | |
Species Reactivity | Human |
Concentration | 1 mg/ml |
Purification |
Alternative Names | |
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Molecular Weight(MW) | 69151 Da |
Function | Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F |
Tissue Specificity | This EIF4B antibody is generated from rabbits immunized with human EIF4B recombinant protein. |
Entrez Gene | 1975 |
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Nucleotide Accession | Q8WYK5 |
Western blot analysis of EIF4B Antibody in NCI-H460 cell line lysates (35ug/lane). EIF4B (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human testis tissue reacted with EIF4B Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clini relevance has not been evaluated.
EIF4B Antibody flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application Notes | WB~~1:100~500 IHC~~1:50~100 FC~~1:10~50: |
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Form | Liquid |
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Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
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