您的位置：首页 > 技术文献 > 产品说明 > p-eIF4B Antibody (Ser 422) Catalog Number：OM184030
| Product Name | p-eIF4B Antibody (Ser 422) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors ａnd RNA molecules. These interactions are facilitated, in part, by the eukaryotic initiation factor 4 family (eIF4) of proteins that are involved in the early initiation of protein synthesis. eIF4B (eukaryotic translation initiation factor 4B) is a 611 amino acid protein that contains one RNA recognition motif ａnd belongs to the eIF4 protein family. Required for proper mRNA/ribosome binding, eIF4B associates with other eIF4 proteins, such as eIF4A, ａnd promotes the ATP-dependent unwinding activity of ｓelect eukaryotic initiation factors. The gene encoding eIF4B maps to human chromosome 12, which encodes over 1,100 genes ａnd comprises approximately 4.5% of the human genome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| WB:1:500~1:2000 More IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | |
|---|---|
| Tissue Specificity | recommended for detection of Ser 422 phosphorylated eIF4B of m ａnd h origin by WB, IP, IF ａnd IHC(P) |
| Entrez Gene | 1975 |
|---|
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung carcinoma tissue showing cylasmic localization.
Western blot analysis of eIF4B phosphorylation expression in untreated (A) ａnd EGF treated (B) HeLa whole cell lysates.
| Application Notes | WB:1:500~1:2000 More IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol. |
① 凡本网注明"来源：易推广"的所有作品，版权均属于易推广，未经本网授权不得转载、摘编或利用其它方式使用。已获本网授权的作品，应在授权范围内
使用，并注明"来源：易推广"。违者本网将追究相关法律责任。② 本信息由注册会员：上海笃玛生物科技有限公司 发布并且负责版权等法律责任。
易推广客服微信
