标题GAB1 [JU47-19] Catalog Number：OM628991

提供者：上海笃玛生物科技有限公司发布时间：2024/9/14 阅读次数：13次 >>进入该公司展台

Product Name	GAB1 [JU47-19]
Antibody Type	Primary Antibodies
Product description	Growth factor triggering of protein tyrosine kinase receptors induces signals that cascade to the nucleus, activating mitogenic as well as other responses. Critical components of this process include adapter proteins such as Shc, IRS-1 ａnd Gab 1 (GRB-associated binder-1) that lack detectable catalytic activity. These are immediate substrates of receptor tyrosine kinase activity ａnd serve to link activated receptors to downstream signaling components. Whereas Shc has been implicated in signaling by diverse receptor families, IRS-1 serves primarily as the major insulin receptor substrate. Shc ａnd Gab 1 also participate in insulin signaling by linking the insulin receptor to Ras by forming complexes with GRB2 (another adapter protein) ａnd Sos independently of IRS-1. Gab 1 is also thought to be involved in the EGF receptor signaling pathway. More
Immunogen	Recombinant protein

Alternative Names	Gab 1 antibody GAB1 antibody GAB1_HUMAN antibody GRB 2 associated binder 1 antibody GRB 2 associated binding protein 1 antibody GRB2 associated binding protein 1 isoform a antibody GRB2 associated binding protein 1 isoform b antibody GRB2-associated binder 1 antibody GRB2-associated-binding protein 1 antibody Growth factor receptor bound protein 2-associated protein 1 antibody More
Molecular Weight(MW)	77 kDa
Cellular Localization	Cytosol.

SwissProt ID	Q13480

Application

Fig1: Western blot analysis of GAB1 on human small intestine tissue lysate using anti-GAB1 antibody at 1/500 dilution.
Application

Fig2: ICC staining GAB1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application

Fig3: ICC staining GAB1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application

Fig4: ICC staining GAB1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application

Fig5: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-GAB1 antibody. Counter stained with hematoxylin.
Application

Fig6: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-GAB1 antibody. Counter stained with hematoxylin.
Application

Fig7: Flow cytometric analysis of Hela cells with GAB1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Positive Control	Human small intestine tissue lysate, LOVO, MCF-7, SH-SY5Y, human skin tissue, rat epididymis tissue, Hela.
Application Notes	WB:1:500-1:2,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 More

Form	Liquid
Storage Instructions	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer	1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

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