标题RPA1 [D9-C4] Catalog Number：OM627706

提供者：上海笃玛生物科技有限公司发布时间：2024/9/18 阅读次数：12次 >>进入该公司展台

Product Name	RPA1 [D9-C4]
Antibody Type	Primary Antibodies
Product description	RPA plays an important role in DNA replication, recombination ａnd repair. The binding of human RPA (hRPA) to DNA involves molecular polarity in which initial hRPA binding occurs on the 5＇ side of an ssDNA substrate ａnd then extends in the 3＇ direction to create a stably bound hRPA. RPA is a major damage-recognition protein involved in the early stages of nucleotide excision repair. It can also play a role in telomere maintenance. The RPA 70 kDa subunit binds to ssDNA ａnd mediates interactions with many cellular ａnd viral proteins. The DNA binding domain lies in the middle of RPA 70 kDa subunit ａnd comprises two structurally homologous subdomains oriented in tandem. RPA contains a conserved four cysteine-type zinc-finger motif, which mediates the transition of RPA-ssDNA interaction to a stable RPA-ssDNA complex in a redox-dependent manner. More
Immunogen	Recombinant protein

Alternative Names	Dmrpa1 antibody Drosophila Replication Protein A antibody DRPA antibody HSSB antibody Human single stranded DNA binding protein antibody MST075 antibody MSTP075 antibody p70 antibody REPA1 antibody Replication factor A antibody Replication factor A protein 1 antibody Replication protein A 70 kDa DNA-binding subunit antibody Replication protein A 70kDa DNA binding subunit antibody Replication protein A1 70kDa antibody Replication protein A1 antibody RF A antibody RF-A protein 1 antibody RFA antibody RFA1_HUMAN antibody RP A antibody RP-A p70 antibody RPA 70 antibody RPA antibody rpa1 antibody Single stranded binding protein 70 antibody Single-stranded DNA-binding protein antibody More
Molecular Weight(MW)	70 kDa
Cellular Localization	Nucleus.

SwissProt ID	P27694

Application

Fig1: Western blot analysis of RPA70 on human RPA70 recombinant protein using anti-RPA70 antibody at 1/1,000 dilution.
Application

Fig2: Western blot analysis of RPA70 on different cell lysates using anti-RPA70 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: K562 Lane 4: A431 Lane 5: COS-7
Application

Fig3: ICC staining RPA70 (green) ａnd actin filaments (red) in Hela cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application

Fig4: Immunohistochemical analysis of paraffin-embedded colon cancer tissue using anti-RPA70 antibody. Counter stained with hematoxylin.
Application

Fig5: Immunohistochemical analysis of paraffin-embedded prostate tissue using anti-RPA70 antibody. Counter stained with hematoxylin.
Application

Fig6: Flow cytometric analysis of Jurkat cells with RPA70 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

Positive Control	Human RPA1 recombinant protein, Hela, MCF-7, K562, A431, COS-7, colon cancer tissue, prostate tissue, Jurkat.
Application Notes	WB:1:500-1:2,000 ICC:1:100-1:500 IHC:1:100-1:500 FC:1:100-1:200 More

Form	Liquid
Storage Instructions	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer	1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.

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