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| Product Name | PRK2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB~~1:100~500 IHC~~1:50~100: | |
| Species Reactivity | Human |
| Concentration | 1 mg/ml |
| Purification |
| Gene Synonyms | |
|---|---|
| Alternative Names | More |
| Molecular Weight(MW) | 112035 Da |
| Function | PKC-related serine/threonine-protein kinase ａnd Rho/Rac effector protein that participates in specific signal transduction responses in the cell. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion ａnd transcription activation signaling processes. Phosphorylates CTTN in hyaluronan-induced astrocytes ａnd hence decreases CTTN ability to associate with filamentous actin. Phosphorylates HDAC5, therefore lead to impair HDAC5 import. Direct RhoA target required for the regulation of the maturation of primordial junctions into apical junction formation in bronchial epithelial cells. Required for G2/M phases of the cell cycle progression ａnd abscission during cytokinesis in a ECT2-dependent manner. Stimulates FYN kinase activity that is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. Regulates epithelial bladder cells speed ａnd direction of movement during cell migration ａnd tumor cell invasion. Inhibits Akt pro-survival-induced kinase activity Mediates Rho protein-induced transcriptional activation via the c- fos serum response factor (SRF). Phosphorylates HCV NS5B leading to stimulation of HCV RNA replication More |
| Tissue Specificity | Ubiquitous. Expressed in numerous tumor cell lines, especially in bladder tumor cells |
| Cellular Localization | Cylasm. Nucleus. Membrane (By similarity). Cell projection, lamellipodium. Cylasm, cytoskeleton. Cleavage furrow. Midbody. Cell junction Note=Colocalizes with PTPN13 in lamellipodia-like structures, regions of large actin turnover. Accumulates during telophase at the cleavage furrow ａnd concentrates finally around the midbody in cytokinesis. Recruited to nascent cell-cell contacts at the apical surface of cells. In the course of viral infection, colocalizes with HCV NS5B at perinuclear region in the cylasm |
| Entrez Gene | 5586 |
|---|
Western blot analysis of anti-PRK2 Pab in placenta lysate. PRK2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Formalin-fixed ａnd paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
| Application Notes | WB~~1:100~500 IHC~~1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high ａnd low pH buffers ａnd neutralized immediately, followed by dialysis against PBS. |
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