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| Product Name | Fibrinogen gamma antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Fibrinogen gamma. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. : | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, BovinePig (84%), Bovine (83%) |
| Concentration | 1 mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | More |
|---|---|
| Molecular Weight(MW) | 52 kDa (note) (2) |
| Cellular Localization | Secreted |
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, ａnd the membrane was blotted with Fibrinogen gamma antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, ａnd the membrane was blotted with Fibrinogen gamma antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Fibrinogen gamma antibody detects Fibrinogen gamma protein by western blot analysis. Human plasma ａnd HepG2 conditioned medium were separated by 10% SDS-PAGE, ａnd the membrane was blotted with Fibrinogen gamma antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Fibrinogen gamma antibody detects FGG protein by western blot analysis. A. 30 µg HepG2 whole cell lysate/extract 10% SDS-PAGE Fibrinogen gamma antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Fibrinogen gamma antibody detects FGG protein at cytosol on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. Fibrinogen gamma antibody dilution: 1:250.
Fibrinogen gamma antibody detects Fibrinogen gamma protein at cylasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Fibrinogen gamma protein stained by Fibrinogen gamma antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.
Immunoprecipitation of Fibrinogen gamma protein from HepG2 whole cell extracts using 5 μg of Fibrinogen gamma antibody . Western blot analysis was performed using Fibrinogen gamma antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
| Positive Control | HepG2 , human plasma , HepG2 sup. , mouse plasma , rat plasma |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. : |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
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